| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,044,257 | A→G | 100% | V227V (GTA→GTG) | yqiK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,044,257 | 0 | A | G | 85.2% | 91.3 / 5.1 | 53 | V227V (GTA→GTG) | yqiK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base G (34/11); total (41/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTG > minE/2044214‑2044312 | cAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATc > 1:540795/1‑69 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:2469348/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:873387/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:1478995/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:3221523/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:1755652/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:3023459/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:3002907/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:2374282/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:225590/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:1022997/69‑1 (MQ=255) gcgAACGTAACGAAGTTGAACAGGATGTGGAAGCCGCAGTGCGTGAGAAAAACCGTGATGCACTTTcgc < 1:186851/69‑1 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:3319535/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:534172/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:420559/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:379699/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2873206/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2934590/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:3159343/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:3056765/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:3092223/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:3113674/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:587205/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2563649/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2210772/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2195939/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2165656/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2121326/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:614931/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:800275/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:1171446/1‑45 (MQ=255) cGAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcg > 1:1501528/1‑45 (MQ=255) agAAGTTGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACcgt > 1:2227568/2‑45 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCTCAAGTTGGAGATTg > 1:235295/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:345994/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:959056/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:961334/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2753194/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:303857/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2956381/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2872005/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2676581/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2656473/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2654327/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:2635116/1‑69 (MQ=255) ttGAACAGGATGTGGAAGTCGCAGTGCGTGAGAAAAACCGTGATGCACTTTCGCGCAAGTTGGAGATTg > 1:103551/1‑69 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:533079/1‑67 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:2585797/1‑67 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:2105802/1‑67 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:1939834/1‑67 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:928756/1‑67 (MQ=255) gAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg > 1:1247106/1‑67 (MQ=255) aGGATGTAGAAGTTGCGGTGCGTGAAAAAAATcgc < 1:1452943/35‑1 (MQ=255) | CAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTG > minE/2044214‑2044312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |