| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,048,393 | T→A | 57.4% | I654I (ATA→ATT) | glnE ← | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,048,393 | 0 | T | A | 57.4% | 32.2 / 73.6 | 75 | I654I (ATA→ATT) | glnE | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base T (7/25); new base A (21/22); total (28/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TTCAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATC > minE/2048328‑2048461 | ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:920192/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:230055/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:2561774/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:2711222/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:2960682/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:3014913/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:3221935/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:1284164/68‑1 (MQ=255)ttcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:3243515/68‑1 (MQ=255) tcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:1369453/67‑1 (MQ=255) tcAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt < 1:1497143/67‑1 (MQ=255) tGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCt < 1:1796917/69‑1 (MQ=255) tGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCt < 1:1425484/69‑1 (MQ=255) gATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCt > 1:324944/1‑68 (MQ=255) gATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCt > 1:1520055/1‑68 (MQ=255) cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:1899982/1‑62 (MQ=255) cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2257283/1‑62 (MQ=255) cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2285506/1‑62 (MQ=255) cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2589637/1‑62 (MQ=255) cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:3417518/1‑62 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:1363158/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:225619/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:526614/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:3030340/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:798184/69‑1 (MQ=255) cACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcatc < 1:1211350/69‑1 (MQ=255) cACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcatc < 1:2677948/69‑1 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCCGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1035152/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGt > 1:557717/1‑68 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGt > 1:2763407/1‑68 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:774058/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:54384/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:2667972/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:79342/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3362151/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:707662/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:375993/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3051575/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3053368/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1017763/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:2142928/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:161020/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1106486/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1795185/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1145093/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1486834/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1972423/1‑69 (MQ=255) gctgAACGACGGCATAAATCATGGCTTCTgccagcc > 1:915682/1‑36 (MQ=25) cgacgGCATCAATCATGGCTTCTGCCTGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2046597/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTATGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2186120/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGctt < 1:828415/67‑3 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGctt < 1:824536/67‑3 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1674021/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1003756/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:3312304/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1492154/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1408550/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1274165/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:736548/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:76363/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1096839/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:859703/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:880724/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:3115017/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:185212/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1963672/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2177871/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2564342/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2856938/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1999568/67‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:3063976/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:2194756/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:15399/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:2834993/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:778655/69‑1 (MQ=255) tATCATGGCTTCCGCCAGCCATGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGatat > 1:1645787/1‑68 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACtt > 1:2793191/1‑41 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2826521/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1994869/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1049681/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2744846/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2830049/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2034765/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1365893/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2885224/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1729657/1‑69 (MQ=255) | TTCAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATC > minE/2048328‑2048461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |