| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,048,405 | C→T | 48.8% | A650A (GCG→GCA) | glnE ← | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,048,405 | 0 | C | T | 48.8% | 2.1 / 77.9 | 88 | A650A (GCG→GCA) | glnE | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base C (32/13); new base T (21/22); total (53/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
CGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGC > minE/2048347‑2048470 | cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2589637/1‑62 (MQ=255)cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:3417518/1‑62 (MQ=255)cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2285506/1‑62 (MQ=255)cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:1899982/1‑62 (MQ=255)cGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcc > 1:2257283/1‑62 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:798184/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:3030340/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:1363158/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:526614/69‑1 (MQ=255) aTTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTc < 1:225619/69‑1 (MQ=255) cACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcatc < 1:1211350/69‑1 (MQ=255) cACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcatc < 1:2677948/69‑1 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCCGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1035152/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGt > 1:557717/1‑68 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGt > 1:2763407/1‑68 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1017763/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:2667972/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:2142928/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3051575/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3053368/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:3362151/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:375993/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:54384/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:707662/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:774058/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:79342/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1972423/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1486834/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:161020/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1145093/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1106486/1‑69 (MQ=255) gctgAACGACGGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTa > 1:1795185/1‑69 (MQ=255) gctgAACGACGGCATAAATCATGGCTTCTgccagcc > 1:915682/1‑36 (MQ=25) cgacgGCATCAATCATGGCTTCTGCCTGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2046597/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTATGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2186120/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGctt < 1:828415/67‑3 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGctt < 1:824536/67‑3 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2177871/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1492154/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1999568/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1408550/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1274165/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2856938/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:736548/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:3115017/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:880724/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:76363/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1096839/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1674021/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:3312304/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1003756/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:2564342/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:1963672/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:185212/67‑1 (MQ=255) cgacgGCATCAATCATGGCTTCTGCCAGCCAGGTTAAGTGATCGCTCACTTTCATTACCGGTAGCGt < 1:859703/67‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:778655/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:2834993/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:2194756/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:15399/69‑1 (MQ=255) cATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGa < 1:3063976/69‑1 (MQ=255) tATCATGGCTTCCGCCAGCCATGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGatat > 1:1645787/1‑68 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACtt > 1:2793191/1‑41 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1365893/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1049681/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1994869/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2034765/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:1729657/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2885224/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2744846/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2830049/1‑69 (MQ=255) tATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc > 1:2826521/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATGGCTCACTATCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:1252922/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACttt > 1:1701330/1‑38 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcat > 1:976507/1‑41 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:354815/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:2643899/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:2866634/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:434717/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:384548/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg > 1:3064092/1‑47 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:678449/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:2662691/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:2257296/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:1947898/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:869957/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:2176015/1‑69 (MQ=255) aTGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt > 1:2974410/1‑69 (MQ=255) ttCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTgcggc < 1:2757371/69‑1 (MQ=255) | CGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGC > minE/2048347‑2048470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |