| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,049,641 | G→A | 18.5% | S238S (AGC→AGT) | glnE ← | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,049,641 | 0 | G | A | 18.5% | 90.2 / 11.2 | 59 | S238S (AGC→AGT) | glnE | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base G (22/26); new base A (4/7); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATC > minE/2049580‑2049709 | ccATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGc < 1:2610900/68‑1 (MQ=255)ccATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGc < 1:273689/68‑1 (MQ=255)ccATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGc < 1:276642/68‑1 (MQ=255)ccATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGc < 1:2817222/68‑1 (MQ=255) aTCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAGCGCCGCAAAACTCAGCAcc > 1:124946/1‑69 (MQ=255) aTCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAGCGCCGCAAAACTCAGCAcc > 1:2715330/1‑69 (MQ=255) aTCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAGCGCCGCAAAACTCAGCAcc > 1:617744/1‑69 (MQ=255) aTCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAGCGCCGCAAAACTCAGCAc > 1:1706349/1‑68 (MQ=255) cgcgTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAg > 1:2022140/1‑69 (MQ=255) cgcgTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAg > 1:3314475/1‑69 (MQ=255) cgcgTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAg > 1:2968977/1‑69 (MQ=255) tAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCg > 1:2894397/1‑67 (MQ=255) tAACGCTCCCAGTCGCGACCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCgg > 1:2208460/1‑68 (MQ=255) cTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACt < 1:3311155/69‑1 (MQ=255) cTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACt < 1:1808892/69‑1 (MQ=255) gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaaa > 1:1744495/1‑69 (MQ=255) gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaaa > 1:2946800/1‑69 (MQ=255) gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaaa > 1:117676/1‑69 (MQ=255) gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaaa > 1:1825954/1‑69 (MQ=255) gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaaa > 1:1838952/1‑69 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:284085/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:2861112/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:2864829/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:3124067/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:2372972/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:3236446/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:1051644/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:1559897/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:683957/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:1388499/69‑1 (MQ=255) gcCCCTGCTCCTGGTAATAATCTTACAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACg < 1:434920/69‑1 (MQ=255) cTCCTGGTAATAATCTTCCAACGGGGCAAAGCTCAGCAcc > 1:1845994/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:3402165/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:753493/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:358342/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:2747700/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:3354211/1‑40 (MQ=255) cTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAcc > 1:1318150/1‑40 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1530856/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1123328/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1317451/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1367662/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1441562/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:2088288/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1582458/68‑1 (MQ=255) ccTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGc < 1:1745430/68‑1 (MQ=255) taataaTCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCata > 1:3366045/1‑69 (MQ=255) taataaTCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCata > 1:3405475/1‑69 (MQ=255) taataaTCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCata > 1:866715/1‑69 (MQ=255) taataaTCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCata > 1:1096827/1‑69 (MQ=255) taataaTCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCata > 1:1818196/1‑69 (MQ=255) atCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGcc < 1:1090748/36‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:3062011/68‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:1515502/68‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:508876/68‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:174997/68‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:391174/68‑1 (MQ=255) cgCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:2785825/68‑1 (MQ=255) gCAAAACTCAGCACCAGCGGGCCACTTTCACCAAACGGACGCAGCCGCATATCCACGCGATAGACGaa < 1:567579/68‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:451583/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:1897664/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:2110360/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:1484962/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:734801/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:778602/69‑1 (MQ=255) gCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATc < 1:3083736/69‑1 (MQ=255) | CCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATC > minE/2049580‑2049709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |