| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,051,274 | A→G | 38.8% | G135G (GGT→GGC) | ygiF ← | predicted adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,051,274 | 0 | A | G | 38.8% | 22.3 / 33.8 | 54 | G135G (GGT→GGC) | ygiF | predicted adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (21/12); new base G (17/4); total (38/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
ACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGC > minE/2051208‑2051335 | acaGATAGGTTCAGCAAATTCACCTGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:141769/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1069712/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:3302555/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:3411451/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:2880294/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:355722/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:2593774/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:386948/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:2275023/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1692752/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1631197/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:643829/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:64669/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:800986/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1121760/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1188374/1‑69 (MQ=255)acaGATAGGTTCAGCAAATTCACCCGCTTTCACCTCACCCTGATCGAAAGCGATTTCAATTCGGCTGcc > 1:1192466/1‑69 (MQ=255) aTAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCg < 1:776189/69‑1 (MQ=255) aTAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCg < 1:2818720/69‑1 (MQ=255) aTAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCg < 1:2691007/69‑1 (MQ=255) aTAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCg < 1:2069227/69‑1 (MQ=255) tAGGTTCAGCAAATTCACCCGCTTTCACTTCCGCCTGGTCGAGGGCGATTTCATTTTGGCTACCATCg < 1:2476411/68‑1 (MQ=255) tCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTAc > 1:1029775/1‑58 (MQ=255) tCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTAc > 1:1622564/1‑58 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGATGGCGATTTCAATTTGGCTACCATCGACCGc < 1:1456758/63‑1 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCa > 1:406751/1‑55 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCg < 1:1095256/58‑1 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc > 1:1524311/1‑63 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc > 1:472628/1‑63 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc > 1:1180990/1‑63 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc < 1:1370725/63‑1 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc < 1:864649/63‑1 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc > 1:279252/1‑63 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc < 1:1583177/63‑1 (MQ=255) aaaTTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGc < 1:1553547/63‑1 (MQ=255) aCTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGGCATTTTTCGCGATa > 1:2828906/1‑68 (MQ=255) aCTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTcgc > 1:985674/1‑64 (MQ=255) aCTTCACCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGa > 1:363322/1‑66 (MQ=255) cccTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATc < 1:1547458/68‑1 (MQ=255) gTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc > 1:2756139/1‑68 (MQ=255) gTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc > 1:671826/1‑68 (MQ=255) gTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc > 1:2452604/1‑68 (MQ=255) gTCGAGGGCGAGTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTcgcg > 1:2571096/1‑54 (MQ=255) ggCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAg > 1:1081808/1‑69 (MQ=255) cAATTAGGCTGCCATCGACCTCTACTAGCCATTTTTCGCGATAAAAATc < 1:749035/49‑1 (MQ=255) cAATTAGGCTGCCATCGACCTCTACTAGCCATTTTTCGCGATAAAAATc < 1:1439456/49‑1 (MQ=255) cAATTAGGCTGCCATCGACCTCTACTAGCCATTTTTCGCGATAAAAATc < 1:1430719/49‑1 (MQ=255) aaTTAGGCTGCCATCGACCTCTACTAGCCATTTTTCGCGATAAAAATc < 1:1540575/48‑1 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:1866976/1‑68 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:1557773/1‑68 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:365556/1‑68 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:2960959/1‑68 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:707086/1‑68 (MQ=255) ttGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc > 1:838012/1‑68 (MQ=255) | ACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGC > minE/2051208‑2051335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |