| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,052,195 | C→T | 46.9% | R92R (CGC→CGT) | htrG → | predicted signal transduction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,052,195 | 0 | C | T | 46.9% | 9.3 / 69.2 | 83 | R92R (CGC→CGT) | htrG | predicted signal transduction protein |
| Reads supporting (aligned to +/- strand): ref base C (29/15); new base T (20/19); total (49/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
GAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACA > minE/2052129‑2052262 | gAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTc > 1:1854520/1‑69 (MQ=255)gAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTc > 1:552795/1‑69 (MQ=255)gAAAGACAGCTCTGGCCGTACCG‑CTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTcc > 1:994272/1‑69 (MQ=255) gACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgt > 1:176999/1‑69 (MQ=255) gACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgt > 1:2093513/1‑69 (MQ=255) gACAGCTCTGGCCGTACCGCCTGGATCCCGGTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgt > 1:474715/1‑69 (MQ=255) aCAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgtg > 1:2047982/1‑69 (MQ=255) aCAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgt > 1:921722/1‑68 (MQ=255) tctGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgtgt > 1:401198/1‑65 (MQ=255) tctGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCgtgt > 1:1751437/1‑65 (MQ=255) gTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTg > 1:837957/1‑68 (MQ=255) aCCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGaa < 1:422412/69‑1 (MQ=255) aCCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGaa < 1:1358895/69‑1 (MQ=255) aCCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGaa < 1:2466156/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa > 1:387603/1‑69 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:610702/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:2908005/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:2206296/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:177103/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:2856554/69‑1 (MQ=255) cccGTTGAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCaa < 1:1290993/69‑1 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCaaaa < 1:1156252/68‑1 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCaaaa < 1:578531/68‑1 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCaaa > 1:1545274/1‑67 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:1838190/1‑69 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:889198/1‑69 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:2760166/1‑69 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:3346484/1‑69 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:3369797/1‑69 (MQ=255) gTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAAc > 1:51066/1‑69 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:3062858/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:3271823/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:3314006/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:358589/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:2410528/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:546675/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:1166628/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:1608085/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:649651/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:1963080/45‑1 (MQ=255) gAAACAACTTAGCACGGAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:992142/45‑1 (MQ=255) gAAACAACTTAGCACGGAACCAAGCCTGCGTTCCCGTGTGCCAGa < 1:3139735/45‑1 (MQ=255) gAAACAACTTAGCACGCAGCCAAGCCTGCGTTCCCGTGTGCCAGa < 1:1727733/45‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATaaa < 1:445900/66‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt < 1:472195/68‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt < 1:3286648/68‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt < 1:1993609/68‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt < 1:209767/68‑1 (MQ=255) aCTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt < 1:2118096/68‑1 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAAt > 1:1916895/2‑40 (MQ=38) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTTACCGATAAACTCa > 1:144118/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTc > 1:2690511/2‑67 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:447361/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:1074637/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:1091214/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:885077/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:733284/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:2072938/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:584302/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:1228668/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:472019/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:125483/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:1398410/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:2547223/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:3434548/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:3427648/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:3373493/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:2737829/2‑68 (MQ=255) ggAGCCAAGCCTGCGTTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACGCTGACCGATAAACTCa > 1:3301968/2‑68 (MQ=255) gAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCAcc < 1:3003128/69‑1 (MQ=255) aGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATc > 1:839432/1‑69 (MQ=255) aGCCTGCGCTCCCGTGTGCC‑‑ATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCg > 1:1218929/1‑68 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:3044187/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:1682222/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:3098864/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:3121298/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:85713/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:2847302/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:2017275/1‑57 (MQ=255) ccTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACt > 1:1470837/1‑57 (MQ=255) cTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt < 1:2742606/69‑1 (MQ=255) cTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt < 1:1497412/69‑1 (MQ=255) cTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt < 1:457634/69‑1 (MQ=255) cTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAaca < 1:1014060/68‑1 (MQ=255) cTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAaca < 1:293917/68‑1 (MQ=255) cTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAaca < 1:2498641/68‑1 (MQ=255) cTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAaca < 1:2544401/68‑1 (MQ=255) | GAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACA > minE/2052129‑2052262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |