| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,055,817 | C→T | 34.7% | S137S (AGC→AGT) | ygiH → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,055,817 | 0 | C | T | 34.7% | 49.8 / 37.1 | 69 | S137S (AGC→AGT) | ygiH | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (21/24); new base T (12/12); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTGGCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGCTCCGTTTTATGTCTGGT > minE/2055757‑2055875 | cATTGGCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtc > 1:3400322/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:1401601/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:1405559/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:3332377/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:2528028/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:249900/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:2251928/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:475520/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa > 1:797030/1‑60 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:3071646/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:2754235/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:318449/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:2629734/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:1823934/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:142943/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACtcgtcg > 1:1828692/1‑69 (MQ=255) ggCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACacgtcg > 1:2638422/1‑69 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:2447108/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:3135082/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:1423011/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:849409/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:832350/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:2273603/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:2189688/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:795715/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:749122/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:710416/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:2077511/1‑68 (MQ=255) tGGGATCTCACTGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCt > 1:174272/1‑68 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:2094080/56‑1 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:1441060/56‑1 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:3329730/56‑1 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:814160/56‑1 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:238781/56‑1 (MQ=255) gggATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGa < 1:310409/56‑1 (MQ=255) cGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCg < 1:1177922/64‑1 (MQ=255) cGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGt < 1:836159/69‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:530114/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:3318899/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:3187731/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:3158805/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:2645916/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:145582/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:1897561/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:2740448/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:2733523/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:2039528/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:2561117/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGTGGTTACTCGTCGCTGGGAg < 1:212609/60‑1 (MQ=255) gAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCg < 1:2840428/62‑1 (MQ=255) aGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAg < 1:947564/69‑1 (MQ=255) aGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAg < 1:2056211/69‑1 (MQ=255) aGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAg < 1:2531208/69‑1 (MQ=255) tAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTg < 1:1680962/69‑1 (MQ=255) tAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTg < 1:574679/69‑1 (MQ=255) tAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTg < 1:1031254/69‑1 (MQ=255) aTGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCa > 1:77883/1‑69 (MQ=255) gggAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGc < 1:2412437/51‑1 (MQ=255) gggAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGc < 1:448528/51‑1 (MQ=255) gggAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGc < 1:759022/51‑1 (MQ=255) tGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGc > 1:3402614/1‑65 (MQ=255) ggTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTc < 1:2662297/50‑1 (MQ=255) ggTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTc < 1:2772425/50‑1 (MQ=255) ggTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTc < 1:17576/50‑1 (MQ=255) ggTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTc < 1:1469088/50‑1 (MQ=255) ggTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTc < 1:2793245/50‑1 (MQ=255) tACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGCTCCGttt < 1:1845432/68‑1 (MQ=255) tGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGCTCCGTTTTATGTCtggt > 1:2147265/1‑69 (MQ=255) tGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGCTCCGTTTTATGTCtggt > 1:2961927/1‑69 (MQ=255) | CATTGGCTGGGATCTCACCGGAGTAATGGCGGGAACCTGGTTACTGACCGTGCTATTGAGCGGATACTCGTCGCTGGGAGCGATTGTCAGTGCACTGATTGCTCCGTTTTATGTCTGGT > minE/2055757‑2055875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |