| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,310 | G→A | 35.6% | T45T (ACG→ACA) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,310 | 0 | G | A | 35.6% | 37.0 / 30.1 | 56 | T45T (ACG→ACA) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/28); new base A (12/8); total (20/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01 | |||||||||||
GATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTG > minE/2057247‑2057368 | gATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtc < 1:1065132/69‑1 (MQ=255) tttCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa > 1:2759009/1‑69 (MQ=255) ccGTATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:3265024/62‑1 (MQ=255) ccGAATGCCTGATGACGTGGTGTATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:2357833/62‑1 (MQ=255) ccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:3247832/62‑1 (MQ=255) ccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:2584717/62‑1 (MQ=255) ccGAATGCCCGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:2664474/62‑1 (MQ=255) cGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:945990/61‑1 (MQ=255) cGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa < 1:2900060/61‑1 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGAt > 1:2176399/1‑68 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:1078419/69‑1 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:3339071/1‑69 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:3313533/69‑1 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2681250/1‑69 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2678908/1‑69 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2133974/1‑69 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:1971809/1‑69 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:2002868/69‑1 (MQ=255) gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:1112123/1‑69 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:2186976/66‑1 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:2646539/66‑1 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:1729130/66‑1 (MQ=255) tAAAACAGCTAATGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:145860/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGtt > 1:2314110/1‑61 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:448849/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:3385945/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:1192590/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:619726/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:3243969/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2932323/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2715345/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2704720/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:701881/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2350392/1‑62 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACGACATGCAAAAAGCGa < 1:2295570/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3171202/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2137091/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2210459/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2263454/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1750540/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3169532/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1945227/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:163602/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:676031/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2739394/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:923352/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:753770/68‑1 (MQ=255) gATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3374817/67‑1 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:457099/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:986892/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1235983/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1381529/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1892023/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:193624/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:2531897/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:2292540/69‑3 (MQ=255) cGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2564279/62‑1 (MQ=255) gtcgtcGATGGGGATAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1649993/56‑1 (MQ=255) | GATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTG > minE/2057247‑2057368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |