| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,322 | G→A | 29.3% | G49G (GGG→GGA) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,322 | 0 | G | A | 29.3% | 68.7 / 28.0 | 68 | G49G (GGG→GGA) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/40); new base A (12/8); total (20/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
GTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTG > minE/2057275‑2057387 | gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGAt > 1:2176399/1‑68 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:3339071/1‑69 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:1112123/1‑69 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2133974/1‑69 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:2002868/69‑1 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:1971809/1‑69 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:3313533/69‑1 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg < 1:1078419/69‑1 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2681250/1‑69 (MQ=255)gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg > 1:2678908/1‑69 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:2646539/66‑1 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:2186976/66‑1 (MQ=255) aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTc < 1:1729130/66‑1 (MQ=255) tAAAACAGCTAATGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:145860/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGtt > 1:2314110/1‑61 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2715345/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2932323/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:3385945/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:1192590/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:619726/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2704720/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:2350392/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:701881/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:448849/1‑62 (MQ=255) tAAAACAGCTAAAGGATGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTc > 1:3243969/1‑62 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACGACATGCAAAAAGCGa < 1:2295570/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2739394/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3171202/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3169532/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2263454/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2210459/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2137091/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1945227/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:923352/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:753770/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1750540/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:676031/68‑1 (MQ=255) ggATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:163602/68‑1 (MQ=255) gATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:3374817/67‑1 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:2292540/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:986892/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1235983/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1381529/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:457099/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:1892023/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:2531897/69‑3 (MQ=255) aTGCCGAAACATCGTCGATGGGAAAAATCATCTACCACACGATGTTCGATAACATGCAAAAAGCGAtcg < 1:193624/69‑3 (MQ=255) cGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:2564279/62‑1 (MQ=255) gtcgtcGATGGGGATAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1649993/56‑1 (MQ=255) tcgtcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGt < 1:13653/69‑1 (MQ=255) tcgtcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGt < 1:42215/69‑1 (MQ=255) tcgtcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGt < 1:3377644/69‑1 (MQ=255) gtcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc > 1:1523254/1‑69 (MQ=255) tcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa < 1:1676378/52‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:1089296/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:575733/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:339634/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:2223423/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:329106/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:2323538/69‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:3156365/68‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:794749/68‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:3028901/68‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:2649686/68‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:1823255/68‑1 (MQ=255) cgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcct < 1:38067/68‑1 (MQ=255) gATTGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:464905/68‑1 (MQ=255) gATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg < 1:1218020/68‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:186368/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:2901710/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:2205985/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:2195571/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:820131/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:920520/69‑1 (MQ=255) ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg < 1:3374923/69‑1 (MQ=255) | GTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTG > minE/2057275‑2057387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |