| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,922 | C→T | 32.2% | T249T (ACC→ACT) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,922 | 0 | C | T | 32.2% | 55.4 / 30.8 | 65 | T249T (ACC→ACT) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/15); new base T (3/18); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
CTGGAGCTACGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACC > minE/2057863‑2057979 | cTGGAGCTACGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAg > 1:686729/1‑68 (MQ=255)cTGGAGCTACACCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAg > 1:1817304/1‑68 (MQ=255) gCTACGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:2993972/69‑1 (MQ=255) gCTACGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:3027739/69‑1 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGTTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:1906263/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:1312905/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:88868/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:2336444/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:81259/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:2083182/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:2070749/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:1123545/1‑69 (MQ=255) aCGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:2043545/1‑69 (MQ=255) cGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGAt > 1:2506833/1‑68 (MQ=255) cGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATg > 1:79444/1‑69 (MQ=255) agaagGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTg > 1:1401822/1‑58 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCa > 1:1306305/1‑52 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:2570271/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:3119595/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:1608447/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:2406906/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:3126094/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:2594243/54‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:622608/64‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:3079529/64‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:725164/64‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2587907/1‑68 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2030258/1‑68 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2255448/1‑68 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:1043503/1‑68 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACa < 1:469689/66‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACa < 1:1390057/66‑1 (MQ=255) agGACTCAACCGTCTGGGGATTGCTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:3282851/54‑1 (MQ=255) agGACCCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGt < 1:3399436/54‑1 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTgccgcc > 1:1198852/1‑68 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3318407/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3379849/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:95821/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3038221/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2449759/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1645533/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1902571/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2970442/1‑69 (MQ=255) tGGGGAATGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:441496/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:445190/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:1068570/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGG‑AACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:2082076/1‑68 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:602792/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2733263/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1329164/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1484657/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:802147/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1516947/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:756073/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3107909/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1744864/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2439877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3211413/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:546725/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2395462/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3159477/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1968877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2051014/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2126044/69‑1 (MQ=255) cTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1964533/63‑1 (MQ=255) | CTGGAGCTACGCCTGGAAGAAGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACC > minE/2057863‑2057979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |