| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,946 | A→G | 47.8% | V257V (GTA→GTG) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,946 | 0 | A | G | 47.8% | ‑2.8 / 38.7 | 46 | V257V (GTA→GTG) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/10); new base G (3/19); total (17/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
AGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGC > minE/2057883‑2058013 | agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:725164/64‑1 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:3079529/64‑1 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTa < 1:622608/64‑1 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:1043503/1‑68 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2255448/1‑68 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2030258/1‑68 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACata > 1:2587907/1‑68 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACa < 1:469689/66‑1 (MQ=255)agGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACa < 1:1390057/66‑1 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTgccgcc > 1:1198852/1‑68 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1645533/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3379849/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3318407/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3038221/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1902571/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2970442/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2449759/1‑69 (MQ=255) tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:95821/1‑69 (MQ=255) tGGGGAATGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:441496/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:445190/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:1068570/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGG‑AACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:2082076/1‑68 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2126044/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:802147/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:756073/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1329164/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1484657/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:602792/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:546725/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1516947/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1744864/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3211413/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3159477/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3107909/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2733263/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1968877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2439877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2395462/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2051014/69‑1 (MQ=255) cTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1964533/63‑1 (MQ=255) ggCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCt < 1:175529/69‑1 (MQ=255) ggCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCt < 1:1746616/69‑1 (MQ=255) aGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3048091/51‑1 (MQ=38) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTgg < 1:3046662/69‑1 (MQ=255) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTgg < 1:1808226/69‑1 (MQ=255) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGCCGTTGCTGTCTCTACCGGCTGCTgg < 1:744930/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1597352/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1548813/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1145446/69‑1 (MQ=255) aCATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1963326/68‑1 (MQ=255) | AGGACTCAACCGTCTGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGC > minE/2057883‑2058013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |