| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,964 | C→T | 36.0% | A263A (GCC→GCT) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,964 | 0 | C | T | 36.0% | 40.1 / 33.6 | 61 | A263A (GCC→GCT) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (14/25); new base T (3/19); total (17/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGG > minE/2057897‑2058023 | tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTgccgcc > 1:1198852/1‑68 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:95821/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3379849/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3318407/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:3038221/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2970442/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:2449759/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1902571/1‑69 (MQ=255)tGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:1645533/1‑69 (MQ=255)tGGGGAATGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGccgccc > 1:441496/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:1068570/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:445190/1‑69 (MQ=255) tGGTCCACAAGGGCTGACTGG‑AACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATcc > 1:2082076/1‑68 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:602792/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2126044/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1329164/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2051014/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3211413/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1968877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2733263/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:546725/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1484657/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2395462/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:2439877/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3159477/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1744864/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:756073/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1516947/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:802147/69‑1 (MQ=255) caAGGGCTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3107909/69‑1 (MQ=255) cTGACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:1964533/63‑1 (MQ=255) ggCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCt < 1:175529/69‑1 (MQ=255) ggCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCt < 1:1746616/69‑1 (MQ=255) aGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCTCGCCATCCGTCAAcc < 1:3048091/51‑1 (MQ=38) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTgg < 1:3046662/69‑1 (MQ=255) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTgg < 1:1808226/69‑1 (MQ=255) ggCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGCCGTTGCTGTCTCTACCGGCTGCTgg < 1:744930/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1145446/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1597352/69‑1 (MQ=255) tACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1548813/69‑1 (MQ=255) aCATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGgcgc < 1:1963326/68‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2541438/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2946525/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:3197523/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:530806/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:702090/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2086797/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:1598758/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:1731203/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2784236/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:1787143/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2481488/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:1928408/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:1444467/47‑1 (MQ=255) atCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:2872006/47‑1 (MQ=255) tCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCt > 1:3201020/1‑41 (MQ=255) tCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGtctc < 1:532275/46‑1 (MQ=255) tCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCAtcgtc > 1:1183067/1‑69 (MQ=255) tCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCAtcgtc > 1:3141471/1‑69 (MQ=255) tCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCAtcgtc > 1:1066989/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:1892725/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:1840650/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:2225108/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:201074/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:1168086/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:2334850/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:1336036/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTcgc > 1:299456/1‑69 (MQ=255) gAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCCgctg > 1:2249358/1‑53 (MQ=255) aTCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCgg > 1:1356779/1‑69 (MQ=255) tCTGCCGCCCGCCATCCGTCAACCTTCGGCGTTGCTGtctc < 1:2660152/41‑1 (MQ=255) | TGGGGATTGGTCCACAAGGGCTGACCGGCAACAGTTCAGTGATGGGCGTACATATCGAATCTGCCGCCCGCCATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGG > minE/2057897‑2058023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |