| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,058,356 | T→C | 56.9% | I91I (ATT→ATC) | ttdB → | L‑tartrate dehydratase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,058,356 | 0 | T | C | 56.9% | 13.1 / 36.2 | 51 | I91I (ATT→ATC) | ttdB | L‑tartrate dehydratase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (10/12); new base C (20/9); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01 | |||||||||||
TGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCG > minE/2058291‑2058404 | tGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAg < 1:2427866/69‑1 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGGGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2314170/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCgg > 1:2423756/3‑67 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1960843/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2515730/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2438618/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2401962/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1037922/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2052860/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:2979171/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:302303/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:3052377/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1839672/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1149435/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:412068/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1171428/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1753332/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1688499/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:1580866/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:3176810/3‑68 (MQ=255) tgtcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGc > 1:473946/3‑68 (MQ=255) tCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa < 1:2756728/66‑1 (MQ=255) aCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa < 1:496492/55‑1 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACTGGCGTGAAACTGGTGGTTGGCaa > 1:2347759/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:1676470/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:2440257/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:2198845/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:2804500/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:1990395/1‑69 (MQ=255) cAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa > 1:3316311/1‑69 (MQ=255) tGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa > 1:550015/1‑48 (MQ=255) tGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggt > 1:2376512/1‑68 (MQ=255) tGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggt > 1:1918359/1‑68 (MQ=255) cGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt < 1:698276/69‑1 (MQ=255) cGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt < 1:1810567/69‑1 (MQ=255) cGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt < 1:1800980/69‑1 (MQ=255) cGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt < 1:1796937/69‑1 (MQ=255) cGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg < 1:2004873/68‑1 (MQ=255) aGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg < 1:2369633/59‑1 (MQ=255) aGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg < 1:1860590/59‑1 (MQ=255) aGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg < 1:319431/59‑1 (MQ=255) aGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg < 1:145846/59‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:899520/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:2512113/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:784600/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:13947/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:3341077/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:3116664/69‑1 (MQ=255) aGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:2135314/69‑1 (MQ=255) gTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:1652453/68‑1 (MQ=255) gTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCCg < 1:2590862/68‑1 (MQ=255) | TGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCG > minE/2058291‑2058404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |