| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,067,040 | T→C | 29.0% | K55K (AAA→AAG) | yqjH ← | predicted siderophore interacting protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,067,040 | 0 | T | C | 29.0% | 84.2 / 38.4 | 86 | K55K (AAA→AAG) | yqjH | predicted siderophore interacting protein |
| Reads supporting (aligned to +/- strand): ref base T (35/26); new base C (19/6); total (54/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAA > minE/2066980‑2067101 | gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:3061887/69‑1 (MQ=255)gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:49423/69‑1 (MQ=255)gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:173203/69‑1 (MQ=255)gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:2987085/69‑1 (MQ=255)gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:741750/69‑1 (MQ=255)gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:2355654/69‑1 (MQ=255)gATGCCCTCTTCAGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:586333/69‑1 (MQ=255) aTGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:169165/68‑1 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:235317/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:825597/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:790484/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:768125/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:1904596/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:3324863/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:576271/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:3025988/1‑68 (MQ=255) ctctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGt > 1:3109872/1‑68 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1902313/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1810514/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:373616/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:277129/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1753308/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1744987/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:531835/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1587899/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:597710/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:91041/67‑1 (MQ=255) gTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGc < 1:1011321/67‑1 (MQ=255) gTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGa > 1:1357891/1‑68 (MQ=255) gTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGa > 1:282568/1‑68 (MQ=255) ggcgtcACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgtg < 1:2854951/47‑1 (MQ=255) agtgagtgTCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:2590153/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCtgt > 1:1069864/1‑36 (MQ=16) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTTATCGTCAAAGCCGCGCGATGTaa > 1:1806214/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:3259281/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:551254/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:85450/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:886790/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:889580/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:936224/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1109421/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:2006998/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:220288/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1802781/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1703368/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:2350046/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:240370/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1232864/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1203107/1‑60 (MQ=255) aGTGAGAGCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:439305/1‑60 (MQ=255) tCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcg > 1:236402/1‑69 (MQ=255) aGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcgcc > 1:1968067/1‑69 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:1348374/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:770758/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:867961/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:1453730/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:386997/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:2216858/69‑1 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAgg > 1:1876973/1‑68 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAgg > 1:1399632/1‑68 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAgg > 1:1275533/1‑68 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGACGCCGAgg > 1:2883148/1‑68 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:63133/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:1561267/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:519573/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:1765239/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:411595/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:20667/69‑1 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcgccgc > 1:433389/1‑61 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2720657/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2740915/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2575059/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:238486/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1156844/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2464048/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1560990/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1578203/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1597664/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2884252/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2971613/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:3382185/1‑69 (MQ=255) aagaagAGTATGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2453807/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:15992/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:2470781/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:3377203/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:321234/1‑69 (MQ=255) | GATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAA > minE/2066980‑2067101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |