| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,067,061 | A→G | 38.2% | R48R (CGT→CGC) | yqjH ← | predicted siderophore interacting protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,067,061 | 0 | A | G | 38.2% | 59.8 / 77.1 | 115 | R48R (CGT→CGC) | yqjH | predicted siderophore interacting protein |
| Reads supporting (aligned to +/- strand): ref base A (35/36); new base G (38/6); total (73/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.50e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
GTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTC > minE/2066999‑2067129 | gTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGa > 1:282568/1‑68 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTTATCGTCAAAGCCGCGCGATGTaa > 1:1806214/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1802781/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1109421/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1703368/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1203107/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:1232864/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:889580/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:2006998/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:2350046/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:886790/1‑60 (MQ=255) agtgagtgCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:240370/1‑60 (MQ=255) aGTGAGAGCCTGGTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTaa > 1:439305/1‑60 (MQ=255) tCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcg > 1:236402/1‑69 (MQ=255) aGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcgcc > 1:1968067/1‑69 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:867961/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:1453730/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:770758/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:1348374/69‑1 (MQ=255) ggTTGAGGAAAGAAGAGCTTGCTGTGATCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTcgccg < 1:2216858/69‑1 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAgg > 1:1399632/1‑68 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAgg > 1:1275533/1‑68 (MQ=255) gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGACGCCGAgg > 1:2883148/1‑68 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:1561267/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:20667/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:63133/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:1765239/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:519573/69‑1 (MQ=255) aaaGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGAc < 1:411595/69‑1 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcgccgc > 1:433389/1‑61 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2720657/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:3382185/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2575059/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2884252/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1597664/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:1578203/1‑69 (MQ=255) aagaagAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2464048/1‑69 (MQ=255) aagaagAGTATGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACa > 1:2453807/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:321234/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:2470781/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:15992/1‑69 (MQ=255) agaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACaa > 1:3377203/1‑69 (MQ=255) tgATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCg > 1:3066796/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGcc > 1:730865/1‑37 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGa > 1:3093800/1‑50 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCgg > 1:671073/1‑68 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:698383/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:3359354/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:1293349/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:303298/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:624367/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:755215/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:856016/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:2726691/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:1012230/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:2640051/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:2239593/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:895952/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:787924/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:2031646/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:1664281/1‑69 (MQ=255) aTCGTCAAAGCCGCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGgc > 1:180641/1‑69 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2854668/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3389561/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:490821/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:376017/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1030672/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1086404/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3121054/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3041233/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1144652/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:632123/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2929388/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1897533/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1548398/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2374722/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:844737/69‑1 (MQ=255) gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2131518/69‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:78021/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2704261/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3131173/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3075496/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1247169/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:3008563/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1630763/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:1631401/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:2339553/67‑1 (MQ=255) cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCt < 1:415335/67‑1 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGa > 1:774860/1‑68 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:2472862/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:1015605/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:1368421/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:2833538/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:2974504/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:2978340/1‑69 (MQ=255) aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt > 1:370735/1‑69 (MQ=255) aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATg < 1:266080/68‑1 (MQ=255) aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATg < 1:1482697/68‑1 (MQ=255) aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTc < 1:1539182/69‑1 (MQ=255) aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTc < 1:3014326/69‑1 (MQ=255) | GTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTC > minE/2066999‑2067129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |