| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 171,738 | C→T | 58.5% | D14D (GAC→GAT) | frr → | ribosome recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 171,738 | 0 | C | T | 58.5% | 9.2 / 28.3 | 41 | D14D (GAC→GAT) | frr | ribosome recycling factor |
| Reads supporting (aligned to +/- strand): ref base C (11/6); new base T (4/20); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
GAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTG > minE/171671‑171804 | gagaCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACa < 1:210131/69‑1 (MQ=255)gagaCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACa < 1:1863868/69‑1 (MQ=255) gATATCAGAAACGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:1197914/68‑1 (MQ=255) gATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:2506154/68‑1 (MQ=255) gATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:2273859/68‑1 (MQ=255) gATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:1317677/68‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3126476/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:2534295/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:852257/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:717212/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3078923/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:2323261/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3150629/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3184955/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3232862/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:3382195/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:519035/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:1646443/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:99721/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:1160067/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:143742/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:1465079/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:1491097/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:1720259/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAAt < 1:969057/69‑1 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGATAAATGCGTAGAAGAGTTCAAAACCCAAATCAGCAAAAt < 1:2250717/69‑1 (MQ=255) tGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCTAATCAGCAAAATACGCACGGGTc > 1:8749/1‑68 (MQ=255) tGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCg > 1:612122/1‑69 (MQ=255) tGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCg > 1:3043977/1‑69 (MQ=255) tGCTGAAGTACGCATGGATAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCg > 1:2268958/1‑69 (MQ=255) cGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTccc > 1:3066323/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGc > 1:3318195/1‑45 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACggg > 1:3130582/1‑50 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:261560/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:3388894/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:3129795/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:2897740/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:1071866/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:2276749/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:2272940/1‑69 (MQ=255) gACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg > 1:1075873/1‑69 (MQ=255) | GAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTG > minE/171671‑171804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |