Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,070,105 | G→A | 72.9% | intergenic (‑316/‑75) | aer ← / → ygjG | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,070,105 | 0 | G | A | 72.9% | 98.4 / 37.4 | 92 | intergenic (‑316/‑75) | aer/ygjG | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
Reads supporting (aligned to +/- strand): ref base G (8/17); new base A (40/27); total (48/44) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.09e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
CAAAATAACAACAAATGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATACTTAAATCGG > minE/2070051‑2070173 | cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:3265028/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1628737/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1632603/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1682191/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1693450/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1888012/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:2141354/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:2316557/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:2356503/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:245544/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1086833/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:3298674/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:3388965/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:3402816/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:418438/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:47514/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:781319/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:826156/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:987615/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1519008/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1481463/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1562140/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1419299/66‑1 (MQ=255) cAAAATAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1128694/66‑1 (MQ=255) aaaaTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:3318837/65‑1 (MQ=255) aaaacagcAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2623487/3‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATccc > 1:1389271/1‑67 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCg > 1:2483077/1‑68 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2429916/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:1215365/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:253330/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2554452/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2687268/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:698645/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2867573/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2977403/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:3152735/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:1338413/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:328332/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:3329117/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:195919/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2308871/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:1385413/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2113267/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:11175/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:2002694/1‑69 (MQ=255) aTAACAGCAAATGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGTTTATCCCGa > 1:835165/1‑69 (MQ=255) aaaTGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTcgcg < 1:137587/69‑1 (MQ=255) aaaTGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTcgcg < 1:1157035/69‑1 (MQ=255) aaaTGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTcgcg < 1:3354655/69‑1 (MQ=255) aaaTGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTcgcg < 1:340478/69‑1 (MQ=255) aaaTGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTcgcg < 1:2047333/69‑1 (MQ=255) aTGTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:1959240/52‑1 (MQ=255) gTTAGCAAATAGCATTAAATATTGCGCAAATGATAACCACATTTGTGttt < 1:819363/50‑1 (MQ=255) gATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCgg < 1:314541/69‑1 (MQ=255) aTAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCgg < 1:760456/68‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGGTCGCAGCCGGAGTGGCGCAATc < 1:320808/69‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCaa > 1:2511302/1‑67 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATc < 1:1216766/69‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATc < 1:3369027/69‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATc < 1:3136503/69‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATc < 1:1130996/69‑1 (MQ=255) tatTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATc < 1:1401037/69‑1 (MQ=255) ttGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGgcgc > 1:113945/1‑63 (MQ=255) ttGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATccc > 1:2243890/1‑69 (MQ=255) ttGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATccc > 1:2533204/1‑69 (MQ=255) gCACAAATGATAACCGAATTTGTGTTTATCCCGAtt > 1:1790987/1‑36 (MQ=255) gCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCTCGATCGCAGCCg > 1:3361191/1‑52 (MQ=255) gCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCt > 1:1434946/1‑68 (MQ=255) gCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCt > 1:2698016/1‑68 (MQ=255) acaAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGc < 1:352043/68‑1 (MQ=255) acaAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGc < 1:341707/68‑1 (MQ=255) acaAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGc < 1:703928/68‑1 (MQ=255) aaaTGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGc < 1:1043684/66‑1 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:569191/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:1829677/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:1865510/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:1878785/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:2054511/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:205769/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:2350546/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:756208/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:27214/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:2784632/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:2910474/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:466310/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:3019093/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:36178/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:3238568/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:341037/1‑68 (MQ=255) aTGATAACCACATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATa > 1:3332988/1‑68 (MQ=255) aTGATAACCACATTTGTGTTAATCCCGATTTTCGCGATAGCAGCCGGAGTgg > 1:3283915/1‑52 (MQ=37) gAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATACTTAAATCgg < 1:3367560/69‑1 (MQ=255) | CAAAATAACAACAAATGTTAACAGATAGCATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGCAATACTTAAATCGG > minE/2070051‑2070173 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |