| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,070,836 | G→A | 31.9% | V219V (GTG→GTA) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,070,836 | 0 | G | A | 31.9% | 44.6 / 23.7 | 53 | V219V (GTG→GTA) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (5/31); new base A (6/11); total (11/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGA > minE/2070779‑2070898 | ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:84539/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:2737541/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:2913807/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:1777287/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:1649182/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:319116/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:58380/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:835846/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGc < 1:1188394/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGTCATCTACCGTTTGGc < 1:3149774/67‑1 (MQ=255)ggCGAAATCGACCTTCCGCAAACCTTTTATGCCACTGCTGCCAGGGTTTCGCCATGTACCGTTTGGc < 1:835924/67‑1 (MQ=255) gCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAat < 1:2492461/69‑1 (MQ=255) tATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAat < 1:3265012/43‑1 (MQ=255) gCCGTTACTGCCGGGCTTCCGTCATGTGCCTTTTGGCAATATCGAAGc < 1:428991/48‑1 (MQ=255) aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa > 1:2227414/1‑68 (MQ=255) aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa > 1:1424714/1‑68 (MQ=255) aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa > 1:129595/1‑68 (MQ=255) aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa > 1:1655725/1‑68 (MQ=255) aCTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCa > 1:3134690/1‑68 (MQ=255) gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa < 1:1215032/67‑1 (MQ=255) gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa < 1:2679208/67‑1 (MQ=255) gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa < 1:2758346/67‑1 (MQ=255) gggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaaa < 1:3027795/67‑1 (MQ=255) cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg < 1:2314750/53‑1 (MQ=255) cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg < 1:479324/53‑1 (MQ=255) cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg < 1:1060656/53‑1 (MQ=255) cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg < 1:214828/53‑1 (MQ=255) cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACg < 1:1781301/53‑1 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:2145945/2‑51 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:1297477/2‑51 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:2059532/2‑51 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:2928934/2‑51 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:409526/2‑51 (MQ=255) tcGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGCTCTTAACGa > 1:380957/2‑51 (MQ=255) gTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgat < 1:1373926/68‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1585615/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:664557/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1334454/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:529832/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1411514/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:411499/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1416802/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:3245557/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:2199247/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1595535/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:3072550/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1805537/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1999101/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:2145900/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1015234/69‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatg < 1:3135181/68‑1 (MQ=255) tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatg < 1:3131352/68‑1 (MQ=255) cATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGtgatga < 1:1277115/68‑1 (MQ=255) | GGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGA > minE/2070779‑2070898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |