| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 171,888 | A→T | 32.0% | T64T (ACA→ACT) | frr → | ribosome recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 171,888 | 0 | A | T | 32.0% | 106.3 / 72.5 | 100 | T64T (ACA→ACT) | frr | ribosome recycling factor |
| Reads supporting (aligned to +/- strand): ref base A (21/47); new base T (20/12); total (41/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATT > minE/171828‑171942 | ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:1093497/1‑68 (MQ=255)ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:3272931/1‑68 (MQ=255)ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:243314/1‑68 (MQ=255)ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:1382587/1‑68 (MQ=255)ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:3140011/1‑68 (MQ=255)ttACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGaaaa > 1:762519/1‑68 (MQ=255) gCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAAc > 1:3410631/1‑68 (MQ=255) ccgaggccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCa > 1:158550/1‑61 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:2952908/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1969909/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1700307/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1682760/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1678727/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1553278/1‑69 (MQ=255) ccgacgccgCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGttt > 1:1201523/1‑69 (MQ=255) gcTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTc < 1:612114/69‑1 (MQ=255) gcTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTc < 1:769489/69‑1 (MQ=255) tCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGtc < 1:68391/69‑1 (MQ=255) tCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGtc < 1:1761290/69‑1 (MQ=255) tCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGtc < 1:1770197/69‑1 (MQ=255) tCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGtc < 1:3320011/69‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2153490/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2653435/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:3230568/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2137041/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:3227686/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2298099/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:236037/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2570213/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2920486/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2438953/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2441894/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:1188569/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:674603/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:392360/39‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCg < 1:2623778/68‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCg < 1:3380795/68‑1 (MQ=255) aaGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCg < 1:1544346/68‑1 (MQ=255) gCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 1:2348712/37‑1 (MQ=255) gCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTc < 1:9682/52‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:2075344/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:1269415/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:3116866/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:2483857/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:3165537/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:450298/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:3358045/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:3169482/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:1952265/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:1974482/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:3262090/69‑1 (MQ=255) agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:210265/69‑1 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTg > 1:1097795/1‑58 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:415152/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:2062015/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:2171226/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:3228404/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:3277526/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:3187082/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:1951497/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:3387473/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:3411668/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:1540033/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:302623/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:576172/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:1434331/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:2548950/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:2940480/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:265993/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:828447/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGa > 1:984762/1‑59 (MQ=255) gaagaTTCCCGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTATCCGGCCGTTg > 1:2721586/1‑58 (MQ=39) gaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAt < 1:2738073/68‑1 (MQ=255) agaTTCCCTTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCg > 1:2519894/1‑64 (MQ=255) agaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCg > 1:649397/1‑53 (MQ=255) agaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCg > 1:1922638/1‑64 (MQ=255) agaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCg > 1:2469511/1‑64 (MQ=255) agaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCg > 1:2498470/1‑64 (MQ=255) agaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCg > 1:63889/1‑64 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:2283617/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:2863827/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:1172251/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:1222603/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:1324153/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:598969/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:485452/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:1501044/41‑1 (MQ=255) gaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAAt < 1:2292530/41‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:525355/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:567669/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:2964684/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:2963358/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:648579/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:3078966/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:1731021/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:1296495/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:1289204/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:191127/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:2891540/61‑1 (MQ=255) ccGTACTCTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGAtt < 1:1978938/61‑1 (MQ=255) | TTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATT > minE/171828‑171942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |