| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,071,472 | C→T | 29.4% | A431A (GCC→GCT) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,071,472 | 0 | C | T | 29.4% | 70.3 / 29.4 | 71 | A431A (GCC→GCT) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (32/18); new base T (7/14); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
TATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAA > minE/2071410‑2071534 | tataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg < 1:3298587/69‑1 (MQ=255)tataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg < 1:146647/69‑1 (MQ=255)tataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg < 1:319376/69‑1 (MQ=255)tataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg < 1:1792116/69‑1 (MQ=255)tataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg < 1:1728540/69‑1 (MQ=255) aCTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc > 1:923113/1‑68 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:2188672/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:827110/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:2011994/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:1863135/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:2329145/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:732964/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:1612203/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:537244/68‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:3259106/68‑1 (MQ=255) aGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:2508389/67‑1 (MQ=255) aGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:3083277/67‑1 (MQ=255) aGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:2291533/67‑1 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCa > 1:579053/1‑61 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:634993/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:2902372/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:3349300/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:2320659/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:3352268/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:3387116/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:369900/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:205617/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:1505064/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:842373/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:1026080/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:1512116/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:1781713/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:2056497/1‑68 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAcc > 1:964077/1‑68 (MQ=255) aTGTTCCGCCAGGGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCca > 1:1446023/1‑69 (MQ=255) ttCCGCCAGCGCGTACTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:942785/58‑1 (MQ=255) ccGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgac < 1:626131/69‑1 (MQ=255) ccGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgac < 1:2991447/69‑1 (MQ=255) ccGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgac < 1:714369/69‑1 (MQ=255) gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac < 1:2866502/69‑1 (MQ=255) gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac < 1:1606568/69‑1 (MQ=255) cTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 1:1878232/1‑67 (MQ=255) cTGGTGGCCGGAACACTCAACAACGCTAAAACGATCCGCATTg < 1:1462862/43‑1 (MQ=37) ggtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:1603782/68‑1 (MQ=255) ggtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:917162/68‑1 (MQ=255) ggtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:3070554/68‑1 (MQ=255) gCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCG‑CACTGACACTGACCATTGAACAGtgtg < 1:2941902/69‑1 (MQ=255) cGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg < 1:340585/68‑1 (MQ=255) cTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:420761/1‑69 (MQ=255) cTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:1571769/1‑69 (MQ=255) cTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:2711566/1‑69 (MQ=255) tCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:2843872/54‑1 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:884929/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:1073320/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:1201321/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:3046198/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:1685649/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:2353232/1‑68 (MQ=255) tCAACAACGCTAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg > 1:2194319/1‑68 (MQ=255) tAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCaa < 1:618547/69‑1 (MQ=255) aaCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCaa < 1:2793302/68‑1 (MQ=255) aCGCCAAAACGTTCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:2894181/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCGACTGACACTGACCATTGAACAGtgtg > 1:1469281/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGAGCATTGAACAGtgtg > 1:2525192/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:2396104/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:1705939/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:1798099/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:181102/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:3296773/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:58723/1‑54 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:523205/1‑54 (MQ=255) | TATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAA > minE/2071410‑2071534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |