| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,071,675 | G→A | 58.6% | L96L (CTG→TTG) | ygjH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,071,675 | 0 | G | A | 58.6% | 36.2 / 82.7 | 111 | L96L (CTG→TTG) | ygjH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/12); new base A (52/13); total (86/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGC > minE/2071616‑2071739 | cATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTc < 1:977963/69‑1 (MQ=255) aTCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTc < 1:3294023/68‑1 (MQ=255) cgTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGca < 1:3020198/69‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:3356014/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:2532037/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:2331797/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:938302/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:3159461/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:2128576/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:795067/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:478319/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:483211/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:1581884/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:1479523/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:680614/42‑1 (MQ=255) gCCCGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTc < 1:1538486/42‑1 (MQ=255) ccGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGttt > 1:2894067/1‑57 (MQ=255) ccGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGttt > 1:981210/1‑57 (MQ=255) ccGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGttt > 1:97964/1‑57 (MQ=255) ccGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGttt > 1:2032557/1‑57 (MQ=255) ccGCAGGCATCATCCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGttt > 1:2112334/1‑57 (MQ=255) cGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg < 1:3370753/69‑1 (MQ=255) cGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg < 1:1968902/69‑1 (MQ=255) cGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg < 1:2263531/69‑1 (MQ=255) ggCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg < 1:1717160/69‑1 (MQ=255) ggCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg < 1:232693/69‑1 (MQ=255) ggCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg < 1:863336/69‑1 (MQ=255) ggCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg < 1:3190076/69‑1 (MQ=255) gCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg < 1:3371927/68‑1 (MQ=255) atcatcCGCTCCGTCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatccat > 1:989360/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGt > 1:1970595/1‑40 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGc > 1:1614317/1‑53 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:770344/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:3335086/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2515409/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:460382/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:540951/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:528218/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:944669/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:1036291/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2649839/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2391268/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2103881/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2495692/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:229203/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2301316/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:1838926/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2341144/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:181338/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:1747148/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:1698924/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2408245/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:1658309/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2435312/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:2444916/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGAACACAGCAgcatgcat > 1:2653647/1‑69 (MQ=255) atcatcCGCTCCGGCGTTAACAAAACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAgcatgcat > 1:3432538/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:512336/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:3201713/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:1762244/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:689707/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:1879397/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:630707/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:3296289/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:673820/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:68296/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:2287306/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:2651937/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:2766702/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:3022290/1‑69 (MQ=255) atcCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTcc > 1:2796947/1‑69 (MQ=255) cGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAc > 1:2514901/1‑69 (MQ=255) cGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAc > 1:1497876/1‑69 (MQ=255) cGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAc > 1:1927787/1‑69 (MQ=255) cGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAc > 1:1958432/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:3009656/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:2954479/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:2348418/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:3010800/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:1851758/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:2311447/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:552764/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:556713/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:1565833/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:264822/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACGCTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:2608115/1‑69 (MQ=255) cGCTCCGGCGTTAACAACACACTTTCACTGCCGTCATCGGTTTCCGCACACAGCAGCATGCATTCCGAc > 1:2757512/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:690875/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:563678/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:3422328/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:3179080/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:884973/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:3112557/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:2807271/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:977520/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:1478108/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:141087/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:1310145/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGc > 1:1247890/1‑69 (MQ=255) aaCAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACCTTTCACCACGc > 1:606780/1‑69 (MQ=255) | CATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGC > minE/2071616‑2071739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |