| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,073,250 | T→C | 18.3% | intergenic (+88/‑96) | ebgR → / → ebgA | DNA‑binding transcriptional repressor/cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,073,250 | 0 | T | C | 18.3% | 97.7 / 12.6 | 65 | intergenic (+88/‑96) | ebgR/ebgA | DNA‑binding transcriptional repressor/cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (32/21); new base C (5/7); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATG > minE/2073187‑2073312 | tCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTAc > 1:2315892/1‑69 (MQ=255)tCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTAc > 1:1999557/1‑69 (MQ=255)tCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTAc > 1:797543/1‑69 (MQ=255)tCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTAc > 1:3102719/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:1144448/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:2859674/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:2093650/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:571934/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:1680212/1‑69 (MQ=255) cGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACt > 1:1326664/1‑69 (MQ=255) gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACTCGCTACtt > 1:1720355/1‑69 (MQ=255) gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACTCGCTACtt > 1:1770594/1‑69 (MQ=255) gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACTCGCTACtt > 1:395825/1‑69 (MQ=255) gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACTCGCTACtt > 1:2935008/1‑69 (MQ=255) gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACTCGCTACtt > 1:2879464/1‑69 (MQ=255) cAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAAt > 1:2183685/1‑69 (MQ=255) tgctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAAtttt > 1:884086/1‑69 (MQ=255) ctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCt > 1:574271/1‑45 (MQ=255) gcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTa < 1:2777609/69‑1 (MQ=255) tATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTc > 1:426732/1‑68 (MQ=255) tATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTc > 1:1297965/1‑68 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTacg > 1:2729570/1‑67 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTaca > 1:1152121/1‑68 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTaca > 1:602436/1‑68 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTaca > 1:2275213/1‑68 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTaca > 1:1628693/1‑68 (MQ=255) cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTaca > 1:1771031/1‑68 (MQ=255) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:1337072/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:773769/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:760921/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:2372645/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:630672/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:2537033/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACTCGCTACTTTTTAGTAAAATTTTTACTAAACTCCCCAGCAATTAcaca < 1:3365436/68‑1 (MQ=38) gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACaa < 1:2188648/69‑1 (MQ=255) gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACaa < 1:1964091/69‑1 (MQ=255) gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACaa < 1:2704347/69‑1 (MQ=255) tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:231047/69‑1 (MQ=255) tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:663916/69‑1 (MQ=255) tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:3056787/69‑1 (MQ=255) tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:1681674/69‑1 (MQ=255) tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:3127683/69‑1 (MQ=255) cATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAcc > 1:2242128/1‑68 (MQ=255) cATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAcc > 1:1956150/1‑68 (MQ=255) tttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATg < 1:852266/69‑1 (MQ=255) tttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATg < 1:285467/69‑1 (MQ=255) tttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATg < 1:245293/69‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGc > 1:2730033/1‑42 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGc > 1:3390983/1‑42 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCa > 1:1790293/1‑62 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:2184991/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:2737254/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:3168874/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:529805/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:1703972/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:1852829/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:2504529/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:3097334/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:2920470/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:2187490/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:1545085/63‑1 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:2145850/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc > 1:2737841/1‑63 (MQ=255) tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACAATCAc > 1:44432/1‑63 (MQ=255) tACCTGCTACTTTTTAGT‑AAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:867079/62‑1 (MQ=255) aCCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAc < 1:2094120/62‑1 (MQ=255) | TCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATG > minE/2073187‑2073312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |