BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,081,487	T→C	27.2%	F394F (TTT→TTC)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,081,487	0	T	C	27.2%	120.9 / 45.6	110	F394F (TTT→TTC)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base T (24/56); new base C (15/15); total (39/71)
Fisher's exact test for biased strand distribution p-value = 7.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTT  >  minE/2081421‑2081555

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:757538/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:1828961/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:3107374/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:1867880/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:2808409/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:2220682/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:2616781/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:1309537/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCgg                                                                    >  1:241163/1‑69 (MQ=255)

gtgtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTgg                                                                    >  1:1342361/1‑69 (MQ=255)

   tATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCGGTc                                                                  >  1:900059/1‑68 (MQ=255)

   tATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCGGTc                                                                  >  1:2118432/1‑68 (MQ=255)

   tATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCGGTc                                                                  >  1:8392/1‑68 (MQ=255)

   tATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGACGTGTTCGGTc                                                                  >  1:2993862/1‑68 (MQ=255)

   tATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTc                                                                  >  1:2648149/1‑68 (MQ=255)

   tATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTc                                                                  >  1:1526877/1‑68 (MQ=255)

   tATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTc                                                                  >  1:1686225/1‑68 (MQ=255)

       ggttgtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:3320665/1‑68 (MQ=255)

       ggtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:972131/1‑68 (MQ=255)

       ggtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:2318608/1‑68 (MQ=255)

       ggtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:1566260/1‑68 (MQ=255)

       ggtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:1209940/1‑68 (MQ=255)

       ggtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTaa                                                              >  1:2477678/1‑68 (MQ=255)

                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              >  1:308785/1‑69 (MQ=255)

                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              >  1:1168588/1‑69 (MQ=255)

                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              >  1:1322873/1‑69 (MQ=255)

                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              >  1:3097923/1‑69 (MQ=255)

                             tGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              <  1:2350468/62‑1 (MQ=255)

                             tGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              <  1:3217643/62‑1 (MQ=255)

                             tGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCa                                              <  1:2450578/62‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAAcctg                                <  1:2064335/68‑3 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:1842366/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:1863328/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:1913754/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:163318/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:2742644/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:526850/68‑1 (MQ=255)

                                     gcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCgg                                <  1:2687447/68‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:2413410/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:2715293/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:730705/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:36566/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:3384404/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:295550/69‑1 (MQ=255)

                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:1762067/69‑1 (MQ=255)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:596988/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:609944/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:2927271/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:633913/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:3196470/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:843209/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:130276/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:1429069/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:1652639/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:2521632/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:1961659/35‑1 (MQ=25)

                                             tACGCTCTCTTCTGACGTGTTCGGTCGTAACGAAg                                                         <  1:1711200/35‑1 (MQ=25)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:3172421/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:1058772/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:1265906/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:496727/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:414138/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:1468695/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:3361979/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:3286756/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:3250955/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:1770658/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTa                                     <  1:2245233/55‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:2901415/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:1312523/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:2117610/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:1394615/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:308845/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:1267036/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:2846206/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:1979694/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:787002/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:3114421/68‑1 (MQ=255)

                                             tACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:1771527/68‑1 (MQ=255)

                                              aCGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg                        <  1:3165976/67‑1 (MQ=255)

                                                 ctctctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCTCAtg                    <  1:627973/68‑1 (MQ=255)

                                                              tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATg                            <  1:2190962/47‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggt              <  1:692781/57‑2 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:3427419/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:716304/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:2221133/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:2091800/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:2035492/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:1610775/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:2017336/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:1626550/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAtggctggc              <  1:2972196/57‑1 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:99633/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:398073/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:3129858/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:2551340/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:2386807/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:23045/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt  >  1:1104411/1‑69 (MQ=255)

                                                                  tGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGt   >  1:1282455/1‑68 (MQ=255)

GTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTT  >  minE/2081421‑2081555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`