| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,082,240 | A→G | 42.0% | E129E (GAA→GAG) | exuR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,082,240 | 0 | A | G | 42.0% | 32.1 / 85.4 | 112 | E129E (GAA→GAG) | exuR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (40/25); new base G (21/26); total (61/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
ATATTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCT > minE/2082173‑2082308 | atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:1252138/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:3261901/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:1419982/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:351267/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:2147959/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:453347/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:2834629/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:2400860/1‑69 (MQ=255)atatTGCCGAATTCGCGGCAACTCAGGTAAAGAAACAGGACATCATGAAACTGATGGCCATTCAGGAAc > 1:221414/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAgg > 1:1173371/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAgg > 1:521724/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAgg > 1:2767975/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAgg > 1:730412/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAgg > 1:3350617/1‑69 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAg > 1:1124555/1‑68 (MQ=255) ttGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCACGAAACTGATGGCCATTCAGGAACAgg > 1:3370400/1‑69 (MQ=255) gCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGgcg > 1:3247941/1‑69 (MQ=255) gCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGgcg > 1:3110826/1‑69 (MQ=255) gCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGgcg < 1:1558291/69‑1 (MQ=255) gCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGgcg < 1:1460437/69‑1 (MQ=255) gCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACaa < 1:1056754/69‑1 (MQ=255) gCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACaa < 1:2664441/69‑1 (MQ=255) gAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGAtt > 1:1084624/1‑68 (MQ=255) gAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGAtt > 1:26002/1‑68 (MQ=255) ggACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAAc < 1:2984865/47‑1 (MQ=255) gACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTgg < 1:1596046/69‑1 (MQ=255) gACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTgg < 1:2830627/69‑1 (MQ=255) gACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTgg < 1:1129305/69‑1 (MQ=255) gACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTgg < 1:419140/69‑1 (MQ=255) aCATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTggg < 1:615664/69‑1 (MQ=255) aCATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTggg < 1:2163157/69‑1 (MQ=255) aCATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTggg < 1:581400/69‑1 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGcgcgcg > 1:310430/1‑38 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTggta > 1:2619483/1‑67 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:2909708/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:1077441/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:2571508/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:78802/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:327215/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:493148/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:2975300/1‑69 (MQ=255) catcatGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGa > 1:1425123/1‑69 (MQ=255) atGAAACTGATGTCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCg < 1:897093/58‑1 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGAt > 1:1862770/1‑52 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2860156/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:965862/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2724999/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:3174008/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:3310644/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2654411/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:1943679/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:1674247/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:1343991/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2148985/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:1257925/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2242124/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:2349996/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:119525/1‑62 (MQ=255) gAAATTGATGGCCATTCAGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTggg > 1:1096950/1‑62 (MQ=255) gAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGc < 1:10966/68‑1 (MQ=255) gAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa > 1:1350977/1‑69 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca > 1:803949/1‑69 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca > 1:808419/1‑69 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca < 1:2099290/69‑1 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca > 1:426725/1‑69 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca < 1:365944/69‑1 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca < 1:958035/69‑1 (MQ=255) gATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCca > 1:3244571/1‑69 (MQ=255) aTGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGt > 1:2524044/1‑42 (MQ=255) gCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGc > 1:2955808/1‑58 (MQ=255) cATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTc < 1:1780448/68‑1 (MQ=255) cATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTc < 1:648908/68‑1 (MQ=255) aGGAGCTGGCACGCGGTGAACAATGCTTCCTTGATTCCGAGTGGGATTTGCa < 1:1991855/52‑1 (MQ=255) aGGAGCAGGCACGCGGTTAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:421154/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1237763/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:2750433/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:250911/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:933599/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:923124/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:82005/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:24151/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1113778/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:658570/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1150740/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1275907/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:3013347/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1336637/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:3076281/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:2032147/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1556659/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:3171680/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:1836814/52‑1 (MQ=255) aGGAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTCGCa < 1:2814354/52‑1 (MQ=255) ggAGCAGGCACGCGGTGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCa < 1:3366081/51‑1 (MQ=255) aaCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTg > 1:569257/1‑47 (MQ=255) aaCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTg > 1:2849703/1‑47 (MQ=255) aaCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTg > 1:1131892/1‑47 (MQ=255) aaCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTg > 1:2362827/1‑47 (MQ=255) aaCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTg > 1:1679382/1‑47 (MQ=255) aCAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGctct > 1:3197294/1‑69 (MQ=255) | ATATTGCCGAATTCGCGGCAACTCAGGTAACGAAACAGGACATCATGAAACTGATGGCCATTCAGGAACAGGCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCT > minE/2082173‑2082308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |