| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,082,342 | G→T | 27.9% | V163V (GTG→GTT) | exuR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,082,342 | 0 | G | T | 27.9% | 70.9 / 23.7 | 50 | V163V (GTG→GTT) | exuR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (12/24); new base T (6/8); total (18/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
GGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGA > minE/2082279‑2082382 | ggATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaa > 1:2606150/1‑69 (MQ=255)ggATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaa > 1:2496687/1‑69 (MQ=255)ggATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaa > 1:1535657/1‑69 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:548308/69‑1 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:3365977/69‑1 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:2541927/69‑1 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:252008/69‑1 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:1704071/69‑1 (MQ=255) aTTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGaaaaaa < 1:1875995/69‑1 (MQ=255) ttCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGa > 1:580914/1‑67 (MQ=255) ttCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGa > 1:1940374/1‑67 (MQ=255) caTTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCg < 1:1026269/69‑1 (MQ=255) caTTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCg < 1:552247/69‑1 (MQ=255) caTTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCg < 1:3162196/69‑1 (MQ=255) caTTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCg < 1:2037404/69‑1 (MQ=255) caTTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCg < 1:1222777/69‑1 (MQ=255) cGCTCTGGCGCCGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:355626/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:3221740/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:1099512/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:2820859/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:537797/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:2585935/1‑69 (MQ=255) cGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATAGTGGAAAAAATGTGGACCCAGCGTAGTCATaa > 1:2682324/1‑69 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:3361361/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:3319359/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:1179956/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:3104831/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:1774847/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:399864/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:2263785/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:103534/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:2052676/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:611776/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:729318/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCg < 1:1736509/69‑1 (MQ=255) ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCACAACCCg < 1:437177/69‑1 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTAc > 1:1649776/1‑65 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTgg > 1:1035794/1‑68 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTgg > 1:2829731/1‑68 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTgg > 1:1243649/1‑68 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTgg > 1:171226/1‑68 (MQ=255) cGCAGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGAGTAGTCATAACCCGTACTgg > 1:1022407/1‑68 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:2674201/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:1298200/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:1384280/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:2495946/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:2151505/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:1903596/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGTTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:78585/66‑1 (MQ=255) aGAACTCCGCCCTGGCGGCTATCGCTGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGa < 1:3015072/66‑1 (MQ=255) | GGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGGA > minE/2082279‑2082382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |