| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 172,564 | T→G | 42.9% | G73G (GGT→GGG) | dxr → | 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 172,564 | 0 | T | G | 42.9% | 8.2 / 49.2 | 49 | G73G (GGT→GGG) | dxr | 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase |
| Reads supporting (aligned to +/- strand): ref base T (13/15); new base G (3/18); total (16/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAGGATGTTGATCA > minE/172514‑172632 | gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCtt > 1:2276541/1‑68 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:1322564/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa > 1:1949207/1‑69 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:178375/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:1727479/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:2179582/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa > 1:1413038/1‑69 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:1222656/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:3348370/69‑1 (MQ=255)gACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTa < 1:739433/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:2391087/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:2903241/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:2437067/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:379890/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1994705/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:3111951/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:3038119/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1521330/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1434367/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1414435/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1372262/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:124205/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1174306/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1161065/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:1019702/69‑1 (MQ=255) tGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTg > 1:3255877/1‑68 (MQ=255) tGAAGCGAGTGCGAAACTCCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:2993872/69‑1 (MQ=255) gAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTTAGTgg < 1:3400469/68‑1 (MQ=255) gAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg > 1:3373417/1‑68 (MQ=255) gAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg > 1:1070555/1‑68 (MQ=255) gAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg > 1:3027469/1‑68 (MQ=255) gAAGCGAGTGCGAAACTTCTTAAAATGATGCTACAGCAACAGGGGAGCCGCACCGAAGTCTTAAGTgg < 1:2934539/68‑1 (MQ=255) aCTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCtt > 1:78098/1‑68 (MQ=255) aCTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCtt > 1:2761446/1‑68 (MQ=255) aCTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCtt > 1:2739467/1‑68 (MQ=255) aCTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCtt > 1:249488/1‑68 (MQ=255) ttcttAAAACGATGCTACAGCAACA‑GGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGa < 1:460641/69‑1 (MQ=255) tcttAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGa < 1:2884133/69‑1 (MQ=255) tcttAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGa < 1:2711709/69‑1 (MQ=255) tcttAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGa < 1:361643/69‑1 (MQ=255) gCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAgcgc > 1:2074235/1‑68 (MQ=255) aCAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTg > 1:2521897/1‑68 (MQ=255) aCAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTg > 1:2441369/1‑68 (MQ=255) aCAGCAACAGGGTAGCCGCACCGAAGTCTAAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTg > 1:3267360/1‑68 (MQ=255) gCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAg < 1:2351818/67‑1 (MQ=255) gCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAg < 1:631952/67‑1 (MQ=255) aGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGTTTGAGGATGTTg < 1:2651915/69‑1 (MQ=255) aGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAGGATGttt < 1:1006114/69‑2 (MQ=255) gTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAGGATGTTGATc > 1:1038806/1‑69 (MQ=255) tAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAGGATGTTGATCa > 1:2970285/1‑69 (MQ=255) | GACGATGAAGCGAGTGCGAAACTTCTTAAAACGATGCTACAGCAACAGGGTAGCCGCACCGAAGTCTTAAGTGGGCAACAAGCCGCTTGCGATATGGCAGCGCTTGAGGATGTTGATCA > minE/172514‑172632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |