| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,084,365 | G→A | 45.9% | Q65Q (CAG→CAA) | yqjC → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,084,365 | 0 | G | A | 45.9% | 15.9 / 70.3 | 87 | Q65Q (CAG→CAA) | yqjC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (26/21); new base A (20/20); total (46/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCAGCAAG > minE/2084300‑2084429 | cAGAATCGTATTGACTGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTg < 1:1198837/69‑1 (MQ=255)cAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTg < 1:458589/69‑1 (MQ=255)cAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTg < 1:954789/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGATTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:233545/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATATCCAACTGc < 1:2446626/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2274373/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2978427/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:444324/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:197351/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1845880/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1975839/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:340716/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1693881/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2230575/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1072628/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2328723/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1299831/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:1262850/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:3331708/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2930527/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:3260569/69‑1 (MQ=255) aGAATCGTATTGACGGTCTGAATAAAGCCCCGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:3105257/69‑1 (MQ=255) gAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGc < 1:2497469/68‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:828827/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:241475/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:2450940/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:3380478/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:729833/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:3206997/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:2647364/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:2624354/69‑1 (MQ=255) cGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:2586345/69‑1 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCt > 1:1390091/1‑61 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:1236307/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:1907184/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:242780/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:729940/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:1063630/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:3084471/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:2033710/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:491291/1‑69 (MQ=255) gTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCg > 1:1846234/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaagata > 1:2431892/1‑67 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:3362194/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:3364884/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:3299692/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:258267/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:3369619/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:1469735/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:897562/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:213995/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:315984/1‑69 (MQ=255) aaTAAAGCCCTGAGTGAAGTCCGGGCCAACTGTACAGATAGCCAGCTGCGTGCCGATCATCAGAAGaaa > 1:1729647/1‑69 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCGAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2315483/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCTTGCCGATCATCAGAAGaaa > 1:3372949/1‑65 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:3208301/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:1034198/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:3114978/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:977163/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2989719/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:1185075/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:1692723/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:1811315/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:1940660/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2054961/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2196818/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2332543/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2351042/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2710749/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:290388/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaaa > 1:2931642/1‑66 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaa > 1:2354988/1‑65 (MQ=255) aaGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAACTGCGTGCCGATCATCAGAAGaaa > 1:2294917/1‑65 (MQ=255) gTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGcc < 1:1925922/36‑1 (MQ=255) ccGGGCCAACTTTTCAGATAGCCAGCTGCGTGCCGATCATCAAAAGAAAATCGCAAAGCAGAAAGATGa < 1:478287/69‑1 (MQ=255) ccGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGa < 1:2448540/69‑1 (MQ=255) ccGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGa < 1:841337/69‑1 (MQ=255) ccGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGa < 1:1287290/69‑1 (MQ=255) ccGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGa < 1:3140760/69‑1 (MQ=255) ggCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGt < 1:681253/69‑1 (MQ=255) ggCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGt < 1:104116/69‑1 (MQ=255) ccAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGa < 1:2566515/64‑1 (MQ=255) aTAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCa > 1:600618/1‑67 (MQ=255) tAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCa > 1:2686271/1‑66 (MQ=255) tAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCa > 1:3085968/1‑66 (MQ=255) tAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCa > 1:1099427/1‑66 (MQ=255) gCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCAGCAAg > 1:1599097/1‑69 (MQ=255) | CAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAGCAGAAAGATGAGGTGGCGGAACGCCAGCAAG > minE/2084300‑2084429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |