| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 177,973 | T→G | 28.1% | G407G (GGT→GGG) | yaeT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 177,973 | 0 | T | G | 28.1% | 103.3 / 54.2 | 89 | G407G (GGT→GGG) | yaeT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (40/24); new base G (10/15); total (50/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGG > minE/177906‑178038 | gTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTa > 1:1083834/1‑69 (MQ=255)gTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTa > 1:3324947/1‑69 (MQ=255)gTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTa > 1:2536420/1‑69 (MQ=255) tCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCa > 1:1786649/1‑69 (MQ=255) tCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCa > 1:826936/1‑69 (MQ=255) tCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCa > 1:1842041/1‑69 (MQ=255) tGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAgg > 1:1150598/1‑69 (MQ=255) tGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAgg > 1:2574802/1‑69 (MQ=255) tGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCAAACGTGTTCCGGGTAGCCCGGACCAgg > 1:1836135/1‑69 (MQ=255) aaTCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:2966729/1‑69 (MQ=255) aaTCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:157155/1‑69 (MQ=255) aaTCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:1547743/1‑69 (MQ=255) aaTCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGAGTTCCGGGTa > 1:1813622/1‑55 (MQ=255) tcttCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:1771288/64‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:42324/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:1974715/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:317607/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:2201476/1‑67 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:236915/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:2327488/1‑67 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:2843781/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:777893/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt < 1:2999360/67‑1 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:1192300/1‑67 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:969014/1‑67 (MQ=255) tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGtt > 1:2992433/1‑67 (MQ=255) cGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg > 1:2785167/1‑67 (MQ=255) cGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg > 1:615355/1‑67 (MQ=255) cGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg > 1:1132566/1‑67 (MQ=255) cGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg > 1:1813883/1‑67 (MQ=255) cGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg > 1:130630/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCATGTTGATgtag < 1:970795/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:1860753/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:2777477/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:2946997/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:2497452/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:314451/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:3121122/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:3024372/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:2612424/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:456046/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:1098932/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:1215605/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:1281703/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:781147/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:762292/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:1387229/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:52233/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:484847/69‑3 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:3245894/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:399732/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:1988998/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:2129390/1‑67 (MQ=255) tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag > 1:2213712/1‑67 (MQ=255) gggCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:2215654/68‑3 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:999424/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:547229/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:3405514/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:3178533/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:1213795/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:1312296/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:2572061/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:2519242/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:1435049/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:1858981/1‑68 (MQ=255) tGAAACTGTCGATACCGATACCCAACGTGTTCCGGCTAGCCCGGACCAGGTTGATGTCGTCTACAAgg > 1:1350542/1‑68 (MQ=255) gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAagag < 1:2906997/69‑1 (MQ=255) gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAagag < 1:248297/69‑1 (MQ=255) gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAagag < 1:2242135/69‑1 (MQ=255) gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAagag < 1:1769337/69‑1 (MQ=255) gataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc < 1:1894616/69‑1 (MQ=255) gataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc < 1:1380651/69‑1 (MQ=255) gataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc < 1:160880/69‑1 (MQ=255) gataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc < 1:3406179/69‑1 (MQ=255) gataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc < 1:2858241/69‑1 (MQ=255) cgataccCAACGTGTTCCGGGGAGCCCGGATCAGGTTGATgtag < 1:3105934/44‑3 (MQ=38) gataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACAcc > 1:173507/1‑69 (MQ=255) gataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGACGTCTACAAGGTAAAAGAGCGCAac > 1:2055820/1‑66 (MQ=255) tgtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGc > 1:1312355/1‑64 (MQ=255) tgtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCaa > 1:2347112/1‑69 (MQ=255) tgtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCaa > 1:1308454/1‑69 (MQ=255) tgtTCAGGGTAGACCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAac > 1:786480/1‑55 (MQ=255) gtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc < 1:1924466/69‑1 (MQ=255) gtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc < 1:2075360/69‑1 (MQ=255) gtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc < 1:722760/69‑1 (MQ=255) gtTCCGGGTAGCCCGGACCAGGTAGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc < 1:2986256/69‑1 (MQ=255) ggTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTgg < 1:2965963/68‑1 (MQ=255) ggTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTgg < 1:2829742/68‑1 (MQ=255) ggTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTgc < 1:1214718/68‑2 (MQ=255) | GTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGG > minE/177906‑178038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |