| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,087,622 | G→A | 43.3% | L39L (CTG→CTA) | yhaH → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,087,622 | 0 | G | A | 43.3% | 15.6 / 43.4 | 60 | L39L (CTG→CTA) | yhaH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (23/11); new base A (22/4); total (45/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTA > minE/2087567‑2087686 | aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGt < 1:3400366/69‑1 (MQ=255)aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGt < 1:2964988/69‑1 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTgg > 1:3148102/1‑59 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATaaaa > 1:2711065/1‑65 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:3094440/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:1949418/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:2274625/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:2579286/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:2742936/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:2903084/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:3024045/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:2838256/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:496913/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:555134/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:695515/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:841651/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:904113/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:1528762/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:1276352/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:1317976/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:134443/1‑69 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGt > 1:2024654/1‑68 (MQ=255) agagTACTGGATGTTTATTCTGGTCAACAGCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:3110696/1‑69 (MQ=39) agagTACTGGATGTTTATTCTGCTCAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGtt > 1:611062/1‑69 (MQ=39) tACTGGATGTTTATTCTGGTCAACATTATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:1337240/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:3411998/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:1474854/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:2957935/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:3208072/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:3312598/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:2648139/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:454029/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:2097921/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:862028/1‑69 (MQ=255) tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGc > 1:1126365/1‑69 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTgg > 1:1629040/1‑68 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGc > 1:3033915/1‑69 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGc > 1:3029844/1‑69 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGc > 1:2888437/1‑69 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGc > 1:2221961/1‑69 (MQ=255) ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGc > 1:1611453/1‑69 (MQ=255) tATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAAcgcg > 1:3287471/1‑68 (MQ=255) tATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACgcgc > 1:2597761/1‑69 (MQ=255) ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCt < 1:1680653/68‑1 (MQ=255) ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCt < 1:1624723/68‑1 (MQ=255) gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCtggtgg > 1:2009448/1‑68 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1615437/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1974608/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1208343/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:2239594/69‑1 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAAGGCGATGGTGGCGAAGGCa > 1:51440/1‑69 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGGTGGTGGCGAAGGCa > 1:890512/1‑69 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCa < 1:1027391/69‑1 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCa > 1:2417298/1‑69 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:2853171/68‑1 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:651725/68‑1 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:3366574/68‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:790511/69‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:1487476/69‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCCGACGACaa < 1:1264850/69‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTa < 1:1682146/68‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTa < 1:1399122/68‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTAGCAACGCGCTGGTGTCGAAGGCACCCTGACGACAATCTACGGTa < 1:3045237/68‑1 (MQ=255) ctgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:3395913/58‑1 (MQ=255) | AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTA > minE/2087567‑2087686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |