| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,087,649 | C→T | 42.4% | R48R (CGC→CGT) | yhaH → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,087,649 | 0 | C | T | 42.4% | 10.8 / 26.0 | 40 | R48R (CGC→CGT) | yhaH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (5/18); new base T (13/4); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTC > minE/2087583‑2087716 | tATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAAcgcg > 1:3287471/1‑68 (MQ=255)tATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACgcgc > 1:2597761/1‑69 (MQ=255) ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCt < 1:1624723/68‑1 (MQ=255) ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCt < 1:1680653/68‑1 (MQ=255) gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCtggtgg > 1:2009448/1‑68 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1208343/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:2239594/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1615437/69‑1 (MQ=255) cAACATCATCTTTACGTTCGTGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGTGCCGGTGGCGa < 1:1974608/69‑1 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAAGGCGATGGTGGCGAAGGCa > 1:51440/1‑69 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGGTGGTGGCGAAGGCa > 1:890512/1‑69 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCa < 1:1027391/69‑1 (MQ=255) tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCa > 1:2417298/1‑69 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:651725/68‑1 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:3366574/68‑1 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacg < 1:2853171/68‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:790511/69‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:1487476/69‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCCGACGACaa < 1:1264850/69‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTa < 1:1682146/68‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTa < 1:1399122/68‑1 (MQ=255) gctgctGGATAAAATGTTAGGCTAGCAACGCGCTGGTGTCGAAGGCACCCTGACGACAATCTACGGTa < 1:3045237/68‑1 (MQ=255) ctgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa < 1:3395913/58‑1 (MQ=255) gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg > 1:226455/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGttttt > 1:1156277/1‑67 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTa > 1:545567/1‑68 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:546701/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:642873/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:655074/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:991445/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:2962421/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:2781389/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:2621435/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:2146295/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:1642130/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:1075019/1‑69 (MQ=255) tGTTAGGCTGGCAACGTGCCGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:1071328/1‑69 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:2807834/68‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:411926/68‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:2491862/68‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:1834012/68‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:1558084/68‑1 (MQ=255) gcgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc > 1:1922068/1‑69 (MQ=255) | TATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTC > minE/2087583‑2087716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |