| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,088,530 | T→C | 34.7% | N296S (AAC→AGC) | yhaJ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,088,530 | 0 | T | C | 34.7% | 29.3 / 23.8 | 46 | N296S (AAC→AGC) | yhaJ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (13/17); new base C (5/11); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01 | |||||||||||
TAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAG > minE/2088467‑2088595 | tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:217753/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:540606/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:2029723/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:503209/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:1603090/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:1755699/1‑69 (MQ=255)tAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa > 1:1732910/1‑69 (MQ=255) ttGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGaa > 1:3188100/1‑68 (MQ=255) ttGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt > 1:1416312/1‑69 (MQ=255) ttGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt > 1:1835889/1‑69 (MQ=255) ttGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt > 1:1726482/1‑69 (MQ=255) tCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGacac < 1:667540/69‑1 (MQ=255) tCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGacac < 1:1717713/69‑1 (MQ=255) cccTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAg < 1:462871/68‑1 (MQ=255) cccTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAg < 1:31946/68‑1 (MQ=255) cccTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAg < 1:2444937/68‑1 (MQ=255) cccTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAg < 1:2613862/68‑1 (MQ=255) aaCAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTc < 1:1406345/68‑1 (MQ=255) aaCAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTc < 1:2240388/68‑1 (MQ=255) ataataAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCa < 1:2677064/69‑1 (MQ=255) ataataAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTCCCCCCa < 1:3285322/69‑1 (MQ=255) taataaATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:2204017/69‑1 (MQ=255) aataTTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:469123/64‑1 (MQ=255) aataTTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:3260228/64‑1 (MQ=255) aataTTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:549974/64‑1 (MQ=255) aataTTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:235389/64‑1 (MQ=255) aataTTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:1947124/64‑1 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:2757861/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:1382427/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:503778/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:1484292/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:1485221/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:1699453/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:454171/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:2134144/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:3088635/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGcc < 1:30429/52‑2 (MQ=255) ttattTTCCGCTAAAAAGTTTGGGAATTTCCC‑CAGACACCAGGATTTCGcc < 1:1184250/51‑2 (MQ=255) tattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCCTcc > 1:658538/1‑54 (MQ=255) tattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCCTCCCCCATACTGTCacga > 1:2307818/1‑69 (MQ=255) tattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCCTCCCCCATACTGTCacga > 1:2882405/1‑69 (MQ=255) tattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCCTCCCCCATACTGTCacga > 1:2811477/1‑69 (MQ=255) tattTTCCGCTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCCTCCCCCATACTGTCacga > 1:1046123/1‑69 (MQ=255) ttCCGTTAAAAAGTTTGGGAATTTCCCGCAGCCACCAGGATTTCGCTTCCCCCATACTGTCACGACGcc > 1:2954089/1‑69 (MQ=255) ttCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGcc > 1:2221668/1‑69 (MQ=255) cGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAg < 1:3155856/68‑1 (MQ=255) ttAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAt < 1:128825/50‑1 (MQ=255) | TAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAG > minE/2088467‑2088595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |