| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,089,147 | A→G | 47.8% | A90A (GCT→GCC) | yhaJ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,089,147 | 0 | A | G | 47.8% | ‑3.5 / 38.8 | 46 | A90A (GCT→GCC) | yhaJ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (14/10); new base G (9/13); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01 | |||||||||||
AAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACA > minE/2089079‑2089208 | aaaaaGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgaga > 1:856301/1‑69 (MQ=255)aaaaaGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgaga > 1:2615469/1‑69 (MQ=255)aaaaaGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgaga > 1:1810129/1‑69 (MQ=255)aaaaaGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgaga > 1:1806071/1‑69 (MQ=255) gtCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATc > 1:3055676/1‑68 (MQ=255) gtCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATc > 1:214884/1‑68 (MQ=255) aCCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTc > 1:1174354/1‑69 (MQ=255) aCCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTc > 1:1244449/1‑69 (MQ=255) aCCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTc > 1:2782391/1‑69 (MQ=255) aCCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTc > 1:1474481/1‑69 (MQ=255) aCCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTc > 1:3102362/1‑69 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:3090683/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:2859321/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:2792538/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:2270698/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:1821746/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:1805044/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:1492275/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:1240980/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:638418/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:1149160/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:631676/53‑1 (MQ=255) cacaATGGTGAGATGCGTTTCCCAACCGCGCGCAAGGGCTTCCGCATCggtag < 1:1467250/53‑1 (MQ=255) acaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGGGCTTCCGCATCggtag < 1:3133033/52‑1 (MQ=255) gagaTGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCaa < 1:2330466/69‑1 (MQ=255) gagaTGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCaa < 1:2363986/69‑1 (MQ=255) gagaTGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCaa < 1:771917/69‑1 (MQ=255) agaTGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCaa < 1:1934008/68‑1 (MQ=255) gCGTTTCCCAACCGCGCGCGAGAGATTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgc > 1:553015/1‑69 (MQ=255) gCGTTTCCCAACCGCGCGCGAGAGATTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgc > 1:1305088/1‑69 (MQ=255) cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgcg > 1:2975921/1‑69 (MQ=255) cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgcg > 1:2402004/1‑69 (MQ=255) cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgcg > 1:2514087/1‑69 (MQ=255) cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCAAGCAAAAcgcg > 1:1754153/1‑69 (MQ=255) cGTTTCACAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAAcgcg > 1:436649/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCaa > 1:1596982/1‑53 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:3289212/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:2343090/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:2561324/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:239176/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:2161233/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:1339605/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:1112347/1‑69 (MQ=255) ccGCGCGCGAGGGCTTCCGCATCGGTAGTCAGTTTATCTACGGCTTCCAGCAAAACGCGCCCTCGTTcc > 1:156320/1‑69 (MQ=255) gcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:1109580/69‑1 (MQ=255) gcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:3357385/69‑1 (MQ=255) gcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:1965769/69‑1 (MQ=255) gcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:1667796/69‑1 (MQ=255) gcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:654216/69‑1 (MQ=255) cgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCcaaca < 1:3168339/68‑1 (MQ=255) | AAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACA > minE/2089079‑2089208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |