Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,183 C→T 49.3% G223G (GGG→GGA)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,1830CT49.3% ‑1.0 / 64.8 73G223G (GGG→GGA) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (9/28);  new base T (6/30);  total (15/58)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT  >  minE/2094123‑2094248
                                                            |                                                                 
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1104925/69‑1 (MQ=255)
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:503789/69‑1 (MQ=255)
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1222164/69‑1 (MQ=255)
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1955473/69‑1 (MQ=255)
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1664617/69‑1 (MQ=255)
cAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1978930/69‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGGACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:3357312/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:338951/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1906955/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:3363402/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1973510/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:3186826/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2269733/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:3167120/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2302514/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2443806/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:3082109/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2669896/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2713936/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2987662/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1253748/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1773306/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:664329/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1705329/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1597889/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1581207/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:13478/67‑1 (MQ=255)
   cgcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1359219/67‑1 (MQ=255)
    gcTACGGCACGGCGCGGCACATTGAGCGGACCATGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2999523/66‑1 (MQ=255)
    gcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1787077/66‑1 (MQ=255)
    gcTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2254516/66‑1 (MQ=255)
     cTACGGCACGGCGCGGCTCATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:2306358/65‑1 (MQ=255)
     cTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:1526701/65‑1 (MQ=255)
       acggcacggcGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                     >  1:1999689/1‑68 (MQ=255)
       acggcacggcGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                     >  1:2632050/1‑68 (MQ=255)
       acggcacggcGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                     >  1:931138/1‑68 (MQ=255)
          gcacggcGCGGCACATTGAGCGGACCAGTCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGt                                                   <  1:1170759/67‑1 (MQ=255)
                 gcgGCACATTGAGCTGAACAGGCAGCACGCCTTCAGTATTCTTTCCACGTTCa                                                          <  1:1787278/53‑1 (MQ=255)
                        aTTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCa                                   <  1:1150856/69‑1 (MQ=255)
                         ttGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:3098127/44‑1 (MQ=255)
                         ttGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                           <  1:1218611/44‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:3062892/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:3046066/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:886275/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:507985/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:936325/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:2783719/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:2717536/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:2708676/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:2583385/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:1271375/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:2281291/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:1276518/69‑1 (MQ=255)
                          tGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:1772600/69‑1 (MQ=255)
                           gAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCa                                   <  1:3182024/66‑1 (MQ=255)
                           gAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                                 <  1:1699643/68‑1 (MQ=255)
                                gACCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:313210/38‑1 (MQ=37)
                                 aCCAGGCAGCACGCCTTCAGTATTCATTCCACGTTCa                                                          <  1:8011/37‑1 (MQ=37)
                                    aGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaa                       <  1:2580589/69‑1 (MQ=255)
                                    aGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaa                       <  1:921492/69‑1 (MQ=255)
                                    aGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaa                       <  1:1950943/69‑1 (MQ=255)
                                     ggCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                      >  1:1137421/1‑69 (MQ=255)
                                     ggCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                      >  1:843963/1‑69 (MQ=255)
                                     ggCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                      >  1:412082/1‑69 (MQ=255)
                                     ggCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                      >  1:24604/1‑69 (MQ=255)
                                                       ttCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCt    >  1:1280404/1‑69 (MQ=255)
                                                       ttCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCt    >  1:1811223/1‑69 (MQ=255)
                                                         cATTCCACGTTGAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:2217044/1‑69 (MQ=255)
                                                         cATTCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:451996/1‑69 (MQ=255)
                                                         cATTCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:449539/1‑69 (MQ=255)
                                                         cATTCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:1997133/1‑69 (MQ=255)
                                                         cATTCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:2454509/1‑69 (MQ=255)
                                                         cATTCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACCCTGCGTCAATTTCCGCttt  >  1:2745406/1‑69 (MQ=255)
                                                            |                                                                 
CAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT  >  minE/2094123‑2094248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑