Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,432 A→G 41.2% S140S (AGT→AGC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,4320AG41.2% 33.2 / 71.6 97S140S (AGT→AGC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (39/18);  new base G (6/34);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.50e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAA  >  minE/2094369‑2094494
                                                               |                                                              
gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:1871286/1‑67 (MQ=255)
gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:958175/1‑67 (MQ=255)
gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:76883/1‑67 (MQ=255)
gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:91795/1‑67 (MQ=255)
gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:48654/1‑67 (MQ=255)
gTGCGAAAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                                             >  1:3516/1‑67 (MQ=255)
   cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAg                                                        <  1:955686/69‑1 (MQ=255)
               gtgtTCTTCTTCGACAATAAACACGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTcc                                            >  1:1238601/1‑69 (MQ=255)
                    cttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTcc                                       >  1:2814580/1‑69 (MQ=255)
                      tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                      >  1:1635454/1‑68 (MQ=255)
                      tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                      >  1:3360995/1‑68 (MQ=255)
                      tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                      >  1:1107326/1‑68 (MQ=255)
                                 aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATcc                           >  1:1507674/1‑68 (MQ=255)
                                 aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg                          >  1:2398898/1‑69 (MQ=255)
                                 aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg                          >  1:1875113/1‑69 (MQ=255)
                                 aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg                          >  1:1825959/1‑69 (MQ=255)
                                 aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg                          >  1:1697628/1‑69 (MQ=255)
                                    cgcgccgccgACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:686064/3‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:1564587/1‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:46082/1‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:819857/1‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:1441921/1‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                       >  1:1437765/1‑69 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                        <  1:3354047/68‑1 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                        <  1:589667/68‑1 (MQ=255)
                                    cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                        <  1:138419/68‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:246391/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:3353147/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:2855027/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:2758579/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:309621/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:1361711/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:83338/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:2369673/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:3209237/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:1706839/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:2027384/37‑1 (MQ=255)
                                       gccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:210607/37‑1 (MQ=255)
                                        ccgccgACAGAGTAATAGGTTTTGCTTAATAGctct                                                    <  1:952329/36‑1 (MQ=255)
                                            cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               <  1:1331853/69‑1 (MQ=255)
                                            cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               <  1:2514967/69‑1 (MQ=255)
                                            cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               <  1:525221/69‑1 (MQ=255)
                                                    tAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg                          >  1:2097445/1‑50 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                      >  1:1953073/1‑36 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa       >  1:528470/1‑67 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2907914/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2778490/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:1990958/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2741519/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2740767/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2655572/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:286818/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:1216578/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2582643/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:2519171/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:1373851/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:1469318/1‑68 (MQ=255)
                                                      aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:3181308/1‑68 (MQ=255)
                                                      aTAGGTTATACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg      >  1:785999/1‑68 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:844742/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:2634049/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:2568406/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:733118/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:2292513/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct               >  1:617247/1‑56 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:1167045/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:3273162/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:646309/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:3202808/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:3237058/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:288415/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:1905137/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:190838/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:2196908/69‑1 (MQ=255)
                                                         ggTTTTACTTAATAGCACTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa  <  1:1785724/69‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCTTTCTCAt            <  1:1627852/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:975823/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:1212670/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:1228412/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:131149/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:922217/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:1604675/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2124122/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:222665/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2274870/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2356402/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2408400/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2524017/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2591233/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:370725/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2653741/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2948387/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2996266/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:3351591/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:3322278/57‑1 (MQ=255)
                                                           ttttGCTTAATAGCTCATCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt            <  1:2091694/57‑1 (MQ=255)
                                                              tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                        <  1:1321336/42‑1 (MQ=255)
                                                               |                                                              
GTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAA  >  minE/2094369‑2094494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑