| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,097,921 | C→T | 67.3% | V242I (GTC→ATC) | garL ← | alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,097,921 | 0 | C | T | 67.3% | 42.6 / 29.0 | 58 | V242I (GTC→ATC) | garL | alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (14/5); new base T (17/22); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGA > minE/2097859‑2097989 | ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACg > 1:2562841/1‑64 (MQ=255)ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACg > 1:623677/1‑64 (MQ=255)ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACg > 1:2230860/1‑64 (MQ=255) gttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCgag < 1:3306153/67‑1 (MQ=255) gttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCgag < 1:976501/67‑1 (MQ=255) ggtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGcc > 1:3124601/1‑68 (MQ=255) ggtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGcc > 1:1979741/1‑68 (MQ=255) gtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGa < 1:1559194/69‑1 (MQ=255) gtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGa < 1:1823144/69‑1 (MQ=255) gtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACa > 1:858181/1‑68 (MQ=255) ttttAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCcaca > 1:319387/1‑68 (MQ=255) ttttAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACaa > 1:396805/1‑69 (MQ=255) ttttAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACaa > 1:1193086/1‑69 (MQ=255) ttttAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACaa > 1:2119134/1‑69 (MQ=255) aGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCa > 1:3118493/1‑69 (MQ=255) aGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCa > 1:277335/1‑69 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:2156562/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:2482155/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:2203022/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:542563/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:277093/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:2805958/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:3017684/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:3169717/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1122660/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1941076/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:193402/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1870005/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:799086/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1749663/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:841261/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1641866/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1478178/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCAAAAACGTcgc < 1:1751369/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTCCCGACAGCCACAAACGTcgc < 1:2238422/60‑1 (MQ=255) cAGTTTCTGAGTGGCAGAGCGGAAGATGCCGAGATCGCTACCGACAGCCACAAACGTcgc < 1:662172/60‑1 (MQ=255) tCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGAt < 1:475778/69‑1 (MQ=255) tGAGTGGCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:1737885/53‑1 (MQ=255) gCAGAGCGGATGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:97994/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACCGCCACAAACGTcgca > 1:1196846/1‑47 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:493725/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:879925/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:2692846/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:382977/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:664944/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:3225814/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:3143848/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:3039775/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:1824136/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:1399795/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:1388502/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:1226909/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:1099951/1‑68 (MQ=255) gCAGAGCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAac > 1:130264/1‑64 (MQ=255) gCAGAGAGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTCGCACCCCATTCCAGATAacgacg > 1:2197797/1‑68 (MQ=255) agagCGGAAGATGCCGAGATCGCTGCCGACAGCCACAAACGTcgc < 1:2834954/45‑1 (MQ=255) gACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTc > 1:2763067/1‑69 (MQ=255) gACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTc > 1:2442894/1‑69 (MQ=255) cGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGa > 1:491191/1‑69 (MQ=255) cGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGa > 1:1055495/1‑69 (MQ=255) | TTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGA > minE/2097859‑2097989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |