| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,101,493 | T→C | 29.7% | G375G (GGT→GGC) | garD → | (D)‑galactarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,101,493 | 0 | T | C | 29.7% | 40.8 / 19.0 | 47 | G375G (GGT→GGC) | garD | (D)‑galactarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (8/25); new base C (4/10); total (12/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-02 | |||||||||||
TATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATT > minE/2101431‑2101553 | tATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCa < 1:2302856/69‑1 (MQ=255)tATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCa < 1:2113212/69‑1 (MQ=255) tcAATATGGGAAAAACCGCCCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACg > 1:1472313/1‑69 (MQ=255) tcAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACg > 1:1103659/1‑69 (MQ=255) tcAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACg > 1:3062534/1‑69 (MQ=255) ggAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTag > 1:738908/1‑67 (MQ=255) ggAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTag > 1:3300202/1‑67 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:1255650/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:2692130/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:2698349/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:2973933/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:1723724/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:3150568/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:3359558/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:625679/67‑1 (MQ=255) caaaaCCGACCGCAGCGCCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:1284105/67‑1 (MQ=255) aGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTcc < 1:2978557/69‑1 (MQ=255) gcCAACCCTTCGCGGGGCAACAAAAAGGGCGGCCTCGCCAACGTGGTAaaga < 1:1872603/52‑1 (MQ=255) cccTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTaa < 1:1791841/69‑1 (MQ=255) cccTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTaa < 1:1923480/69‑1 (MQ=255) gcggggCAACAAAAAGGGCGGCCTCGCCAACGTGGTAAAGAAGGCACTCGGCTCCATTGCTAAATCgg > 1:3068996/4‑68 (MQ=255) gcggggCAACAAAAAGGGCGGCCTCGCCAACGTGGTAAAGAAGGCACTCGGCTCCATTGCTAAATCgg > 1:246556/4‑68 (MQ=255) gcggggCAACAAAAAGGGCGGCCTCGCCAACGTGGTAAAGAAGGCACTCGGCTCCATTGCTAAATCgg > 1:2415932/4‑68 (MQ=255) gcggggCAAAAAAAAGGGCGGCCTCGCCAACGTGGTAAAGAAGGCACTCGGCTCCATTGCTAAATCgg > 1:2599369/4‑68 (MQ=255) gggCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCa < 1:1661124/43‑1 (MQ=255) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:2492262/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:2170042/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:454303/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:2720146/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:3374108/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:1978161/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:1556576/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:193720/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:1768426/50‑1 (MQ=38) gaacaaGAAAGGCGGTCTGGCAAATGTGGTGGAGAAGGCACTCGGCTCCAt < 1:3112989/50‑1 (MQ=38) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:98983/69‑1 (MQ=255) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:2384718/69‑1 (MQ=255) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:1666318/69‑1 (MQ=255) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:194294/69‑1 (MQ=255) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:286656/69‑1 (MQ=255) aaGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:2156827/69‑1 (MQ=255) aaaGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTaaa > 1:432610/1‑60 (MQ=255) aaaGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTaaa > 1:1293164/1‑60 (MQ=255) aaaGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTaaa > 1:2289005/1‑60 (MQ=255) aaaGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAAtt < 1:3297728/69‑1 (MQ=255) aaaGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAAtt < 1:522638/69‑1 (MQ=255) ggCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCa < 1:1352523/63‑1 (MQ=255) | TATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATT > minE/2101431‑2101553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |