| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,141 | A→G | 32.8% | A204A (GCT→GCC) | agaR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,141 | 0 | A | G | 32.8% | 121.1 / 83.0 | 149 | A204A (GCT→GCC) | agaR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (27/73); new base G (20/29); total (47/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
TTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCA > minE/2103073‑2103208 | ttGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGa > 1:1726472/1‑69 (MQ=255) tGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAg < 1:874337/69‑1 (MQ=255) cTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGcttc < 1:2939556/69‑1 (MQ=255) cTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGcttc < 1:1102047/69‑1 (MQ=255) cTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGcttc < 1:2321653/69‑1 (MQ=255) ttACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGcttc < 1:2471624/68‑1 (MQ=255) ttACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGcttc < 1:3054437/68‑1 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg > 1:1728034/1‑69 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg < 1:25679/69‑1 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg > 1:2219260/1‑69 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg < 1:338724/69‑1 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg < 1:1898237/69‑1 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg > 1:3408199/1‑69 (MQ=255) ggTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg > 1:1010492/1‑69 (MQ=255) gTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg < 1:2250055/68‑1 (MQ=255) cACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCt < 1:2252355/69‑1 (MQ=255) cACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCt < 1:3200379/69‑1 (MQ=255) cACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCt < 1:1636383/69‑1 (MQ=255) cACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCt < 1:1824745/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:1265679/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:2522510/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:2931917/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:862318/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:2228283/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:2192808/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:2080674/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:626210/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:1522510/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:469188/69‑1 (MQ=255) cGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGa < 1:1357133/69‑1 (MQ=255) tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1855277/1‑68 (MQ=255) tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:361340/1‑68 (MQ=255) tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1600727/1‑68 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:727527/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1933694/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:596629/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:373298/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:177892/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1747303/66‑1 (MQ=255) cacaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGatcgatcg < 1:1572798/67‑1 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:25481/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1384921/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1026456/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1246093/1‑47 (MQ=255) caTCCGA‑GGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:2406546/1‑46 (MQ=39) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCa > 1:1072151/1‑52 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3197591/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:496859/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3089891/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3259067/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3377850/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:891736/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:695217/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:720468/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2460784/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2572461/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2078523/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1375257/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1372482/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2346141/1‑53 (MQ=37) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:3334103/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:641552/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:681932/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:23350/1‑68 (MQ=255) ggTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1459222/58‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:1445303/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:2682094/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3311860/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:1052667/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3341766/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3356325/69‑1 (MQ=255) ttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:2661737/68‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:2310351/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:1574048/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:554498/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:1007121/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:842724/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:324672/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:135974/69‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1142688/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:595449/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1333328/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1665351/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1662008/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:3291636/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:3099546/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAg < 1:2807827/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:456448/69‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:2157430/69‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:2205522/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:975683/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:1024027/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2817396/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2449542/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:773543/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:3049416/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2183564/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:672676/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:659086/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:1634288/69‑1 (MQ=255) | TTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCA > minE/2103073‑2103208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |