| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,167 | C→T | 24.1% | V196I (GTC→ATC) | agaR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,167 | 0 | C | T | 24.1% | 169.6 / 43.2 | 128 | V196I (GTC→ATC) | agaR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (49/48); new base T (19/12); total (68/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
TCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATT > minE/2103118‑2103224 | tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1600727/1‑68 (MQ=255)tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1645134/1‑68 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1933694/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:177892/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1863140/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:596629/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:3011595/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:2185042/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:373298/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:2241559/66‑1 (MQ=255) cacaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGatcgatcg < 1:1150547/67‑1 (MQ=255) cacaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGatcgatcg < 1:1572798/67‑1 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1573585/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:2560158/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1026456/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1459503/1‑47 (MQ=255) caTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:1384921/1‑47 (MQ=255) caTCCGA‑GGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGAcgc > 1:2406546/1‑46 (MQ=39) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCa > 1:1072151/1‑52 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2346141/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2572461/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:695217/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3377850/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3089891/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2078523/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2460784/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:720468/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3197591/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1375257/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1080344/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:891736/1‑53 (MQ=37) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:23350/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:1049361/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:3206967/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:1357226/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:3334103/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:137344/1‑68 (MQ=255) ggTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1459222/58‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:2682094/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3311860/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:1052667/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3356325/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3341766/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:324672/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:1007121/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:2310351/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:554498/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:842724/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:135974/69‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1019265/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1142688/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:595449/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:656072/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:3291636/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1665351/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:709477/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1333328/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAg < 1:2293073/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAg < 1:2807827/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:842730/69‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:2205522/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:773543/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:672676/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:659086/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:3409095/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:574426/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:975683/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:1634288/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2183564/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2449542/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:3049416/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2817396/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:290084/69‑1 (MQ=255) cttcatcttcatTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:2913158/63‑1 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:2797805/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1082061/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1550744/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:2403654/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1736218/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:464697/1‑64 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1267048/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:100531/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1676477/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1784555/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1840664/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2146767/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2261827/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:512596/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:459463/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:408744/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2514623/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2675829/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGt > 1:448509/1‑68 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGt > 1:1147192/1‑68 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:1940604/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:479114/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:1278375/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:2667601/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:3297838/1‑69 (MQ=255) gACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAg < 1:2570533/42‑1 (MQ=255) | TCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATT > minE/2103118‑2103224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |