| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,173 | G→A | 24.1% | R194C (CGC→TGC) | agaR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,173 | 0 | G | A | 24.1% | 167.5 / 43.8 | 128 | R194C (CGC→TGC) | agaR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (49/48); new base A (19/12); total (68/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATT > minE/2103118‑2103224 | tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:361340/1‑68 (MQ=255)tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1855277/1‑68 (MQ=255)tCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat > 1:1600727/1‑68 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:3011595/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:2185042/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:373298/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:2241559/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1933694/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:177892/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:596629/66‑1 (MQ=255) atccaTCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:727527/66‑1 (MQ=255) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCa > 1:1072151/1‑52 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:720468/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:695217/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3089891/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:891736/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2572461/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1372482/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3197591/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3355880/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:1080344/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:3332994/1‑53 (MQ=37) cGACGGTTTAAACGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAg > 1:2346141/1‑53 (MQ=37) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:641552/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:23350/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:3206967/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:137344/1‑68 (MQ=255) cGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTac > 1:681932/1‑68 (MQ=255) ggTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGc < 1:1459222/58‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3341766/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:2682094/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3311860/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:1445303/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:3356325/69‑1 (MQ=255) tttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:1052667/69‑1 (MQ=255) ttAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa < 1:2661737/68‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:324672/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:2310351/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:1007121/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:842724/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:554498/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:135974/69‑1 (MQ=255) tAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGaaa < 1:1574048/69‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1662008/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1142688/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1333328/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1019265/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:3291636/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:656072/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1665351/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:595449/68‑1 (MQ=255) aCGGGCTTCATCTTCATTATGCGTGCTGATGCCGCACTCCAGATCGATCGCATCTACACCAAGAAACa < 1:1959235/68‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:456448/69‑1 (MQ=255) cGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGc < 1:2157430/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:975683/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:3049416/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:773543/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:672676/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:574426/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:3409095/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:290084/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2817396/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:1024027/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:1634288/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2183564/69‑1 (MQ=255) gAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2449542/69‑1 (MQ=255) gCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa < 1:2700385/67‑1 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAgatcga > 1:1135740/1‑40 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:2970636/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:464697/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1736218/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:221640/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1550744/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:229335/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:2403654/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:1279784/1‑64 (MQ=255) tcatcttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCa > 1:2797805/1‑64 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1267048/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1551980/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1065303/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1676477/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:100531/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1784555/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:1840664/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:512596/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:459463/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:408744/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2146767/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2261827/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2422890/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2514623/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2675829/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATc > 1:2849905/1‑42 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGt > 1:1147192/1‑68 (MQ=255) ttcatTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGt > 1:846957/1‑68 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:479114/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:1940604/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:1278375/1‑69 (MQ=255) aTGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt > 1:3297838/1‑69 (MQ=255) gACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAg < 1:2570533/42‑1 (MQ=255) | TCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATT > minE/2103118‑2103224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |