| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,531 | C→T | 46.3% | T74T (ACG→ACA) | agaR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,531 | 0 | C | T | 46.3% | 5.8 / 48.3 | 61 | T74T (ACG→ACA) | agaR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (12/20); new base T (9/19); total (22/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
TCATCGCGGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAG > minE/2103476‑2103599 | tCATCGCGGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAg > 1:2640539/1‑68 (MQ=255) tCGCGGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATc < 1:978597/68‑1 (MQ=255) tCGCGGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATc < 1:2838524/68‑1 (MQ=255) cgcgGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATc < 1:1127829/67‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATGGCAGATCAACGCGccac < 1:2960914/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:2554341/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:906091/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:51036/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:3431649/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:3175162/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:1747773/68‑1 (MQ=255) cAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGccac < 1:1314987/68‑1 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGTTATCGCAGATc > 1:329943/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:1474577/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:11400/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:947485/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:819688/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:3429462/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:2747276/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:1839729/1‑54 (MQ=255) gcgcTTTTATCTTCCACTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATc > 1:1740327/1‑54 (MQ=255) tttATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg < 1:3284981/69‑1 (MQ=255) tttATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg < 1:490366/69‑1 (MQ=255) tttATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg < 1:2269678/69‑1 (MQ=255) tttATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg < 1:607664/69‑1 (MQ=255) tttATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg < 1:698568/69‑1 (MQ=255) tATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg > 1:1359964/1‑67 (MQ=255) tATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg > 1:2444082/1‑67 (MQ=255) tATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg > 1:824196/1‑67 (MQ=255) tATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGgcacg > 1:861892/1‑67 (MQ=255) aTCTTCCACTGATGGCTCGACTGACGGCGTCGTTCTATc > 1:2599739/1‑39 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:611837/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1147484/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1143759/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:910935/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:401605/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1417193/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:3193230/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:3138508/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:124988/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2662335/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2657014/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2336000/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2396972/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2096116/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:2499782/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1988615/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1849616/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1692852/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTTGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATc < 1:1278662/68‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATcc < 1:783908/69‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATcc < 1:1220414/69‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATcc < 1:1646644/69‑1 (MQ=255) cTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATcc < 1:2825327/69‑1 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATcccc > 1:1576453/1‑65 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:2114390/1‑69 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:55411/1‑69 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:1485277/1‑69 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:1836988/1‑69 (MQ=255) gCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:3104072/1‑69 (MQ=255) gCTCGACTGACGGCGTAGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCt > 1:2498658/1‑69 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:2136204/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:1183633/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:1529681/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:719789/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:739501/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:2609856/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:1503393/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:191945/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:309346/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:1226455/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:161861/69‑1 (MQ=255) cgtGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAg < 1:2675958/69‑1 (MQ=255) | TCATCGCGGTGTTCAGTGCGCTTTTATCTTCCACTGATGGCTCGACTGACGGCGTCGTGCTATCGCAGATCAACGCGCCACCATAGGCACGCACAGCGATCCCCTGCTTTTCCAGAAACGCCAG > minE/2103476‑2103599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |