| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,973 | A→G | 26.6% | intergenic (‑221/‑28) | agaR ← / → kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,973 | 0 | A | G | 26.6% | 110.9 / 40.6 | 101 | intergenic (‑221/‑28) | agaR/kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base A (29/45); new base G (10/17); total (39/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
ATCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACA > minE/2103909‑2104034 | aTCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGc < 1:10301/69‑1 (MQ=255)aTCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGc > 1:2796364/1‑69 (MQ=255)aTCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGc > 1:2412112/1‑69 (MQ=255)aTCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGc > 1:2107175/1‑69 (MQ=255)aTCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGc > 1:1713183/1‑69 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:548122/67‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:505169/67‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:365325/67‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:2283374/67‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:226723/67‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:895758/66‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:3322051/66‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:2658146/66‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:2485657/66‑1 (MQ=255) gTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:2235449/66‑1 (MQ=255) ttttatttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTaa < 1:3088780/66‑1 (MQ=255) ttttatttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:2712858/65‑1 (MQ=255) tttatttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:1610025/69‑1 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTg > 1:1813031/1‑66 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:1843005/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:216492/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:2473651/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:2109597/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:636533/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3088070/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3099514/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3259349/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:1665640/1‑69 (MQ=255) ctcACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGagag < 1:1775848/68‑1 (MQ=255) ctcACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGagag < 1:2117834/68‑1 (MQ=255) tcACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGagag < 1:2260889/67‑1 (MQ=255) tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTa < 1:3080285/46‑1 (MQ=255) tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGa < 1:3133266/69‑1 (MQ=255) aTCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:1628543/42‑1 (MQ=255) aTCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:623560/42‑1 (MQ=255) aTCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:1057310/42‑1 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1821138/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:2851656/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1042804/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:3377138/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:109529/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1489849/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAAAATCGCAGTAATCTGAACTGGAGACGAAAGTGa > 1:2828610/1‑59 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1626172/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1405270/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:2666853/1‑68 (MQ=255) aaCTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACa > 1:2120162/1‑68 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:2113233/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1129018/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:428800/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:3082352/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1296346/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:828775/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTCACAGAAAt < 1:405094/69‑1 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAg > 1:2652903/1‑48 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGaaa > 1:2550901/1‑66 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:3318424/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1925025/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:855663/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:863459/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:274795/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1581709/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:2629987/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:544669/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:81524/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:364888/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1499408/1‑68 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:3278962/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:2250933/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:834923/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:1390433/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:3129498/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2000258/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:678456/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:682364/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:362874/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2432733/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:392353/69‑1 (MQ=255) cAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2994852/68‑1 (MQ=255) aaaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:2562034/69‑1 (MQ=255) aaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:1356194/68‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTTGAGAGGAAAGTGAAACATCTGa < 1:589089/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:683162/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:815648/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1017040/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:362911/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:3341640/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:3334556/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2854106/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:280758/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1167746/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2485805/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2064321/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2053313/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1822569/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1704920/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1677422/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGACAGGAAAGTGAAACATCTGa < 1:2259303/50‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAGCATCTGACAGAAATGGTGAGACAGcaca < 1:2672048/69‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGcaca < 1:2862496/69‑1 (MQ=255) | ATCTTTCGTTTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACA > minE/2103909‑2104034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |