| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,985 | T→G | 32.2% | intergenic (‑233/‑16) | agaR ← / → kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,985 | 0 | T | G | 32.2% | 104.7 / 85.5 | 115 | intergenic (‑233/‑16) | agaR/kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/50); new base G (14/23); total (42/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 115 total aligned reads displayed.
TTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAA > minE/2103918‑2104050 | tttatttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:1610025/69‑1 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTg > 1:1813031/1‑66 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3099514/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:1665640/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3259349/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:636533/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:1843005/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:3088070/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:2109597/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:216492/1‑69 (MQ=255) atttttatCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATGTCGCAGTAATCTGAAc > 1:2473651/1‑69 (MQ=255) ctcACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGagag < 1:1775848/68‑1 (MQ=255) tcACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGagag < 1:2260889/67‑1 (MQ=255) tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGa < 1:3133266/69‑1 (MQ=255) aTCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:623560/42‑1 (MQ=255) aTCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTg < 1:1057310/42‑1 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1042804/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:3377138/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:2851656/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:109529/1‑66 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1626172/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1405270/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:2666853/1‑68 (MQ=255) aaCTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACa > 1:2120162/1‑68 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1129018/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:2113233/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:428800/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1296346/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:828775/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTCACAGAAAt < 1:405094/69‑1 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAg > 1:2652903/1‑48 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGaaa > 1:2550901/1‑66 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1925025/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:544669/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:2629987/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1581709/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:274795/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1499408/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:855663/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:81524/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:863459/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:364888/1‑68 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:1390433/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:2250933/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:3278962/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:834923/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:392353/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2432733/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:362874/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2000258/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:3129498/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:678456/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:682364/69‑1 (MQ=255) cAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2994852/68‑1 (MQ=255) aaaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:2562034/69‑1 (MQ=255) aaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:1356194/68‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:3341640/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2854106/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:3334556/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:280758/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1017040/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2485805/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2064321/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2053313/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1822569/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1704920/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1677422/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:683162/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1167746/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGACAGGAAAGTGAAACATCTGa < 1:2259303/50‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAGCATCTGACAGAAATGGTGAGACAGcaca < 1:2672048/69‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGcaca < 1:2862496/69‑1 (MQ=255) tCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:2241919/48‑1 (MQ=255) tCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:441046/50‑1 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2505921/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2795075/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:132500/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2059466/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2029277/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:18997/1‑69 (MQ=255) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:590052/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:880799/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1253804/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1292186/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1303072/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:834600/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1527776/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1550593/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1656407/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1776031/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:184008/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:3319798/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1954829/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2248278/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:397192/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2893400/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2919334/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:342350/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2928095/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:3310104/44‑1 (MQ=38) | TTTATTTTTATCTCACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAA > minE/2103918‑2104050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |