| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 180,434 | A→T | 43.8% | T213T (ACA→ACT) | lpxD → | UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 180,434 | 0 | A | T | 43.8% | 31.5 / 103.8 | 96 | T213T (ACA→ACT) | lpxD | UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (39/15); new base T (23/19); total (62/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
ACTGGGTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGT > minE/180367‑180498 | aCTGGGTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGcaca > 1:2147641/1‑68 (MQ=255)aCTGGGTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGcaca > 1:1830874/1‑68 (MQ=255) gggTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCa < 1:27018/69‑1 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTAcc > 1:341148/1‑65 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:3050273/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:290293/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:334569/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:3403310/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:3431617/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:2451084/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:2352269/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:2009219/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:132401/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCattg > 1:1385693/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCatt > 1:1688981/1‑67 (MQ=255) tGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCatt > 1:2217337/1‑67 (MQ=255) gATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCatcgatcg > 1:901148/1‑69 (MQ=255) gATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCatcgatcg > 1:482447/1‑69 (MQ=255) gATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCatcgatcg > 1:1596479/1‑69 (MQ=255) gATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCatcgatcg > 1:3337088/1‑69 (MQ=255) gATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCatcgatcg > 1:3038574/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:3169239/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:2579199/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1138724/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:3037051/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1373290/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:193672/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1529390/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1657149/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1732922/1‑69 (MQ=255) caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1738860/1‑69 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:312897/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2527436/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2902292/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:3413588/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1050360/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1980159/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2265072/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:912677/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1067336/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2803262/1‑68 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2820240/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2816176/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2921021/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1141003/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:583515/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2967225/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2472972/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:3324218/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2314119/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:61100/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:3054206/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2016581/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:74881/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1729444/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:829731/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:842352/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:78168/69‑1 (MQ=255) gTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGGGCGCTGGAt < 1:76438/68‑1 (MQ=255) cgcgTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGa < 1:3205525/68‑1 (MQ=255) gcgTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGa < 1:2308309/67‑1 (MQ=255) ttGGCGATCGCGTGGAGATCGGCGCCTGCACAACCATCGATCGCGGCGCGCTGGATGac < 1:2772694/59‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:1025820/69‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:2234200/69‑1 (MQ=255) gagaTCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:929586/42‑1 (MQ=37) gaTCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1435639/40‑1 (MQ=37) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:412546/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:566690/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:643254/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:676600/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2683941/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:3018884/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:1122071/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:1455111/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2299700/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2303557/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2410818/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2417533/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2636360/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2683337/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2755024/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2675227/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2823306/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2949576/1‑68 (MQ=255) gCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAAc < 1:2673467/69‑1 (MQ=255) gCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAAc < 1:1550227/69‑1 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1041004/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3259472/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1210569/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:894159/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:865964/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1353266/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:773184/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1762110/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:725867/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:2066593/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:207568/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3373814/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3377935/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCAATCATAAcc > 1:1861576/1‑65 (MQ=255) | ACTGGGTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGT > minE/180367‑180498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |