| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,103,998 | A→C | 31.7% | intergenic (‑246/‑3) | agaR ← / → kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,103,998 | 0 | A | C | 31.7% | 118.0 / 88.7 | 123 | intergenic (‑246/‑3) | agaR/kbaZ | DNA‑binding transcriptional dual regulator/tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base A (28/56); new base C (14/25); total (42/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTA > minE/2103936‑2104059 | tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGa < 1:3133266/69‑1 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1042804/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1489849/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:1821138/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:2851656/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:3377138/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCt > 1:109529/1‑66 (MQ=255) tCAACTGAAACAAAACGAAAGATTAAAATCGCAGTAATCTGAACTGGAGACGAAAGTGa > 1:2828610/1‑59 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:2666853/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1405270/1‑68 (MQ=255) cAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGAc > 1:1626172/1‑68 (MQ=255) aaCTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACa > 1:2120162/1‑68 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1129018/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:3082352/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:1296346/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:2113233/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAAt < 1:828775/69‑1 (MQ=255) gAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTCACAGAAAt < 1:405094/69‑1 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAg > 1:2652903/1‑48 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGaaa > 1:2550901/1‑66 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1499408/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:3318424/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:364888/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:274795/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:2629987/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:544669/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1925025/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:81524/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:863459/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:1581709/1‑68 (MQ=255) aaCAAAACGAAAGATTAATATCGCAGTAATCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATg > 1:855663/1‑68 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:1390433/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:3278962/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:834923/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:2250933/67‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:392353/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:3129498/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:678456/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2432733/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:362874/69‑1 (MQ=255) aCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:682364/69‑1 (MQ=255) cAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:2994852/68‑1 (MQ=255) aaaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:2562034/69‑1 (MQ=255) aaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTga < 1:1356194/68‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:280758/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:683162/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:362911/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1017040/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1704920/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:1677422/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:815648/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:3341640/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGa < 1:2053313/50‑1 (MQ=255) aaGATTAATGTCGCAGTAATCTGAACTGGACAGGAAAGTGAAACATCTGa < 1:2259303/50‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAGCATCTGACAGAAATGGTGAGACAGcaca < 1:2672048/69‑1 (MQ=255) gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGcaca < 1:2862496/69‑1 (MQ=255) tCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATg < 1:2241919/48‑1 (MQ=255) tCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGt < 1:441046/50‑1 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2795075/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:18997/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2029277/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:132500/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2505921/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:234459/1‑69 (MQ=255) aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACaaa > 1:2059466/1‑69 (MQ=255) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:397192/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:342350/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1253804/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:590052/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:880799/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:3310104/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1527776/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1776031/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:184008/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1954829/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2028029/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1303072/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2893400/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:2919334/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:3319798/44‑1 (MQ=38) aTCGGAACTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1289147/44‑1 (MQ=38) gAAGTGGAGATGCAAGTGAAACTTCTGTCAGAAATGGTga < 1:347860/40‑1 (MQ=255) aCTGGAGAGGCAAGTGAAACATCTGACAGAAATGGTga < 1:1260856/38‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:1324421/69‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:1202077/69‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:1428049/69‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:55691/69‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:740295/69‑1 (MQ=255) cTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:341260/69‑1 (MQ=255) tGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:155451/68‑1 (MQ=255) tGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:1360059/68‑1 (MQ=255) tGGAGAGGAAAGTGAAACATCGGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAAtt < 1:378978/68‑1 (MQ=255) tgagaGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:2186585/2‑69 (MQ=255) ggAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:362242/1‑69 (MQ=255) ggAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:1775014/1‑69 (MQ=255) ggAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:1043362/1‑69 (MQ=255) ggAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:1653384/1‑69 (MQ=255) ggAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTa > 1:2756367/1‑69 (MQ=255) ggAGAGGAAAGAGAAACATCTGACAGAAATGGTGAGACAGCACaa > 1:291496/1‑45 (MQ=255) aaaGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACa < 1:47814/51‑1 (MQ=255) | TGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTA > minE/2103936‑2104059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |