| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,105,113 | A→T | 35.3% | V371V (GTA→GTT) | kbaZ → | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,105,113 | 0 | A | T | 35.3% | 71.3 / 68.6 | 85 | V371V (GTA→GTT) | kbaZ | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base A (23/32); new base T (14/16); total (37/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
CGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGT > minE/2105052‑2105178 | cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:96810/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:2510964/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:2685770/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:2021700/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:2836969/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:751523/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:1977068/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:3124354/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:1478582/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:1356686/68‑1 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATc > 1:1588990/1‑69 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATc > 1:2380613/1‑69 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATc > 1:697935/1‑69 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATc > 1:1072511/1‑69 (MQ=255)cGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATc > 1:1325379/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:962014/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:3016641/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:3039739/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:3081919/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:3083343/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:1795605/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:2917874/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:199240/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:1087931/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:1515907/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:376225/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:430670/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:1476830/1‑69 (MQ=255) cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCg > 1:1024919/1‑69 (MQ=255) gTGCGCTATTTCTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:622082/60‑1 (MQ=255) gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:1783497/68‑1 (MQ=255) gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:842721/68‑1 (MQ=255) gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:2751036/68‑1 (MQ=255) gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:1363170/68‑1 (MQ=255) gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:2503841/68‑1 (MQ=255) cTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCAcc < 1:1223570/69‑1 (MQ=255) cTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCAcc < 1:367021/69‑1 (MQ=255) ggCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgc < 1:372500/69‑1 (MQ=255) ggCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgc < 1:2664496/69‑1 (MQ=255) ggCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgc > 1:1503699/1‑69 (MQ=255) cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:1900242/44‑1 (MQ=255) gACAGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAATCTGGCGGATTCACCAATTccgctgccg < 1:1024517/69‑1 (MQ=255) aGCGAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:619725/39‑1 (MQ=38) aGCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:1458037/39‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgcttccgctg < 1:441645/69‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgctgccgctg < 1:2482234/69‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgctgccgctg < 1:3300272/69‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgctgccgctg < 1:1206245/69‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgctgccgctg < 1:2009218/69‑1 (MQ=255) aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTccgctgccgctg < 1:2102521/69‑1 (MQ=255) gCCAGATTGATGACGCTTTCGCTCATCTGGTTCGTAAt < 1:849284/38‑1 (MQ=255) gCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa < 1:1861754/46‑1 (MQ=255) aGATTGATTACGCTTTCGCTCTTCTGGTACGTAATCTGGCGGa < 1:338477/43‑1 (MQ=38) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGa > 1:2798253/1‑40 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:454355/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:1477459/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:563968/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:57623/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:40998/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:3209841/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:1650194/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:312385/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:3063292/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:3059294/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:1892222/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:1337698/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:2024552/1‑48 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:2127852/48‑1 (MQ=255) ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa > 1:1142170/1‑48 (MQ=255) tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCaa < 1:994852/47‑1 (MQ=255) tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGcc > 1:3249313/1‑68 (MQ=255) tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGcc > 1:777078/1‑68 (MQ=255) tgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGt < 1:2430833/68‑1 (MQ=255) tgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGt < 1:1592975/68‑1 (MQ=255) tgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGt < 1:553543/68‑1 (MQ=255) tgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGt < 1:1108994/68‑1 (MQ=255) tgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGt < 1:1901272/68‑1 (MQ=255) gaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTa < 1:3274411/68‑1 (MQ=255) gaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTa < 1:1816371/68‑1 (MQ=255) gaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTa < 1:1494767/68‑1 (MQ=255) cGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc > 1:1946727/1‑68 (MQ=255) cGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc > 1:952031/1‑68 (MQ=255) cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAg > 1:222205/1‑69 (MQ=255) cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAg > 1:3046022/1‑69 (MQ=255) cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAg > 1:101541/1‑69 (MQ=255) aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt < 1:876237/66‑1 (MQ=255) aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt < 1:263167/66‑1 (MQ=255) aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt < 1:1583354/66‑1 (MQ=255) | CGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGT > minE/2105052‑2105178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |