| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,108,247 | T→C | 33.6% | T6T (ACT→ACC) | kbaY → | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,108,247 | 0 | T | C | 33.6% | 89.9 / 67.1 | 119 | T6T (ACT→ACC) | kbaY | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Reads supporting (aligned to +/- strand): ref base T (44/35); new base C (28/12); total (72/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAT > minE/2108185‑2108307 | cAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAatat > 1:806688/1‑69 (MQ=255) attcatCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGt > 1:2645675/1‑61 (MQ=255) catCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGcc < 1:2984303/69‑1 (MQ=255) tGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGcc < 1:1163832/69‑1 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGccc > 1:2278149/1‑55 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCATGCCAATGGcacc > 1:892347/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCATGCCAATGGcacc > 1:379639/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1604758/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1033367/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1979174/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1992694/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:2061014/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:2297560/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:2390787/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:2424098/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:2867388/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:3263974/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:473386/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:712817/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:928125/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:946214/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1577526/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:147214/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:1309475/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcac > 1:3062197/1‑66 (MQ=255) tAAGAGGATCGCATTATGAGCATTATATCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGcacc > 1:848953/1‑66 (MQ=255) aaGATGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:572339/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:2672032/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:1645790/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:1046422/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:1724158/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:286953/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:1212295/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:2474641/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:3395110/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:380021/69‑1 (MQ=255) aaGAGGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:485053/69‑1 (MQ=255) aaGACGATCGCATTATGAGCATTATCTCCACCAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg < 1:1939945/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCTCTAAATATCTGTTACa < 1:1533883/45‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTa < 1:2040244/43‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTa < 1:2983350/43‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTa < 1:1766536/43‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa < 1:2072160/45‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa < 1:2867063/45‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa > 1:3289074/1‑45 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa < 1:901974/45‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa > 1:2373678/1‑45 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACa > 1:2601181/1‑45 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:2790245/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1033753/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:1120923/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:706046/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:3004669/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:3007821/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:702184/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:3181068/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:1157564/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:545164/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:538077/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:1145256/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1729779/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:2722615/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:2660608/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1730335/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:2475959/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1334091/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:2763818/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1461530/1‑69 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:2339946/69‑1 (MQ=255) agagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcg > 1:1523001/1‑68 (MQ=255) gagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTa < 1:974706/42‑1 (MQ=255) gagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:2109571/68‑1 (MQ=255) gagGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:1496736/68‑1 (MQ=255) ggATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGTCCAATGGCTACGCGGt < 1:115376/69‑1 (MQ=255) ggATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGt < 1:399908/69‑1 (MQ=255) ggATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGt < 1:924737/69‑1 (MQ=255) tCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1028017/1‑63 (MQ=255) tCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:2366017/1‑63 (MQ=255) tCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc > 1:1126940/1‑63 (MQ=255) gCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTg < 1:2152597/69‑1 (MQ=255) gCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTg < 1:1607695/69‑1 (MQ=255) aTTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGcc < 1:162800/48‑1 (MQ=255) ttATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAcgc < 1:385209/58‑1 (MQ=255) aTGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCtttt > 1:3003705/1‑69 (MQ=255) aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAAc > 1:941214/1‑69 (MQ=255) aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAAc > 1:1319357/1‑69 (MQ=255) aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAAc > 1:770350/1‑69 (MQ=255) aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAAc > 1:1915122/1‑69 (MQ=255) aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAAc > 1:1364609/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTAc > 1:51706/1‑45 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACAtt > 1:3374060/1‑66 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:1666051/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:2008573/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:7177/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:713564/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:1875535/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:1906540/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:1419765/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:2339985/1‑69 (MQ=255) aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAt > 1:2754540/1‑69 (MQ=255) | CAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCAT > minE/2108185‑2108307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |