| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,108,643 | G→C | 36.5% | L138L (CTG→CTC) | kbaY → | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,108,643 | 0 | G | C | 36.5% | 59.2 / 65.1 | 78 | L138L (CTG→CTC) | kbaY | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/30); new base C (8/20); total (25/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
CTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTA > minE/2108575‑2108710 | cTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTg < 1:1132669/69‑1 (MQ=255)cTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTg < 1:103442/69‑1 (MQ=255)cTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTg < 1:1337947/69‑1 (MQ=255)cTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTg < 1:2138809/69‑1 (MQ=255) gAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCC‑CCTGGGCGgt < 1:1210538/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTTGGCGgt < 1:440964/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:303378/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:463472/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:330574/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:3303127/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:2162041/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:2831868/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:1981984/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:1922503/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:3095063/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:1568452/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt > 1:1558221/1‑69 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:1470811/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGgt < 1:1187335/69‑1 (MQ=255) aaaTCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTAGGCGgt < 1:1979680/69‑1 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:2470910/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:2404850/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:2846449/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:2991590/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:2255627/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:90784/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:1254521/1‑68 (MQ=255) aTCGGTTGTCGACTTCTGCCACTCCCAGGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTCGGCGgtg > 1:1352660/1‑68 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGaa < 1:1575329/68‑1 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGaa < 1:1084740/68‑1 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGaa < 1:559259/68‑1 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGaa < 1:609833/68‑1 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGaa < 1:1565135/68‑1 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAg > 1:347753/1‑69 (MQ=255) ttgttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAg > 1:2426429/1‑69 (MQ=255) gACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa < 1:1269058/68‑1 (MQ=255) gACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa < 1:2006763/68‑1 (MQ=255) gACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa < 1:755041/68‑1 (MQ=255) gACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa < 1:818254/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:2834508/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:1064556/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:247114/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:2393023/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:506496/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:2225265/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:2153834/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:178902/68‑1 (MQ=255) aCTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGtt < 1:1526320/68‑1 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:3285058/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:3261625/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:625085/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:861391/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:953462/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:1177718/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:2638575/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:2875672/1‑55 (MQ=255) cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGa > 1:2060965/1‑55 (MQ=255) gCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTg < 1:748033/69‑1 (MQ=255) gCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTg < 1:395746/69‑1 (MQ=255) gAAGCTGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:3374551/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:489854/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:1204972/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:872635/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:1663172/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:1962695/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:660451/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2033761/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2053746/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:584933/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2282237/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:488612/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:267498/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2738266/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2763336/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2833854/67‑1 (MQ=255) gAAGCAGAACTGGGCCGCCTAGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:434808/67‑1 (MQ=255) aaGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:3378705/66‑1 (MQ=255) aaGCAGAACTGGGCCGCCTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:1693008/66‑1 (MQ=255) cgccTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:235322/52‑1 (MQ=255) ccTCGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTcc < 1:2644062/50‑1 (MQ=255) tGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:3414964/69‑1 (MQ=255) tGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:915564/69‑1 (MQ=255) | CTGGTGAAATCGGTTGTTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTA > minE/2108575‑2108710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |