| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 25,217 | G→A | 52.3% | E83K (GAA→AAA) | rihC → | ribonucleoside hydrolase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 25,217 | 0 | G | A | 52.3% | ‑1.2 / 56.3 | 65 | E83K (GAA→AAA) | rihC | ribonucleoside hydrolase 3 |
| Reads supporting (aligned to +/- strand): ref base G (9/22); new base A (17/17); total (26/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
CCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGT > minE/25159‑25280 | ccACGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAt < 1:3187982/69‑1 (MQ=255)ccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAt < 1:1005002/69‑1 (MQ=255)ccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAt < 1:2595168/69‑1 (MQ=255)ccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAt < 1:1698799/69‑1 (MQ=255) ggCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAtggctg > 1:862950/1‑68 (MQ=255) ggCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAtggctg > 1:3231207/1‑68 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:180640/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:1046992/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2309003/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2661859/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2751259/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2803350/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2966723/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2977338/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:3019928/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:627330/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:761745/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:1787128/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:1538995/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:1640758/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttctt < 1:1462106/68‑1 (MQ=255) tGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCAAATCAGGAATGGCTGGCTACGACTttgtt < 1:3051650/68‑1 (MQ=255) ggTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTttgttg > 1:3385366/1‑68 (MQ=255) ggTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTttgttg > 1:1508272/1‑68 (MQ=255) tGATGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTttgtt < 1:2451733/52‑1 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:3324432/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:3262355/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:623781/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:3104016/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:697446/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:702972/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2865523/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2829172/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:1194181/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2642596/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2052222/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:1803872/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2418380/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2066310/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:2347838/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:225721/1‑68 (MQ=255) tGCGGCATCTGTGCACGGCAAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:268086/1‑68 (MQ=255) gCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCgg < 1:1304067/68‑1 (MQ=255) tCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc > 1:898333/1‑61 (MQ=255) tCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGa > 1:2276542/1‑67 (MQ=255) tCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGa > 1:2960549/1‑67 (MQ=255) tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTttg < 1:1270908/38‑1 (MQ=255) tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTttg < 1:66541/38‑1 (MQ=255) tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTttg < 1:2442067/38‑1 (MQ=255) tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATAcc > 1:1766931/1‑69 (MQ=255) tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATAc > 1:2344162/1‑68 (MQ=255) gtgCACGGCGAATCGGGAATGGCTGGCTACGACTttg < 1:3403412/37‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAACGCCGCTCGGGATACCGGCGt < 1:1900193/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:3296369/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:3211712/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:3202590/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:3167589/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:2977980/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:1523469/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:694321/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:1534256/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:288640/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:1205260/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGt < 1:1719236/68‑1 (MQ=255) cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCGCGGGATACCGGCGt < 1:2289979/68‑1 (MQ=255) | CCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGT > minE/25159‑25280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |