| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,117,769 | C→T | 38.2% | K218K (AAG→AAA) | yraL ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,117,769 | 0 | C | T | 38.2% | 32.6 / 36.3 | 60 | K218K (AAG→AAA) | yraL | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/32); new base T (7/16); total (12/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
TGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTCAG > minE/2117710‑2117831 | tgtgCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTAcc < 1:2842798/66‑1 (MQ=255)tgtgCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTAcc < 1:702984/66‑1 (MQ=255) gtgCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCAc < 1:2959514/68‑1 (MQ=255) tgCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTAcc < 1:554223/64‑1 (MQ=255) cTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGc < 1:2532129/67‑1 (MQ=255) cTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGc < 1:95854/67‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:375520/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:2419477/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:2138174/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:2137635/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:2124668/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:3184271/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:3203401/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:3265722/68‑1 (MQ=255) gACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCagcagc < 1:45833/68‑1 (MQ=255) aCCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTc > 1:1896421/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:1650597/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:1538776/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:1440300/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:1410010/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:2677673/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGcc > 1:2614995/1‑69 (MQ=255) ttCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCTTTTACCCACGCCAGCAGCTCGc > 1:1616722/1‑68 (MQ=255) aTCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACggg > 1:3063467/1‑68 (MQ=255) cAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGgcgc > 1:535230/1‑69 (MQ=255) cAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGgcgc > 1:1024286/1‑69 (MQ=255) cAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGgcg > 1:950510/1‑68 (MQ=255) aGCACCATTTCGCCTTTGCGACTGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:123374/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCATCTCGcc < 1:988964/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:2322525/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:922399/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:2244720/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:2012483/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:905560/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:3046149/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:724284/59‑1 (MQ=255) aGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:1639481/59‑1 (MQ=255) aGCACCATTTCGCCTTAGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:1729891/59‑1 (MQ=255) gCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGcc < 1:1764554/58‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:826162/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:343799/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:1677852/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:1621926/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:1488913/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:1021509/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:1298352/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:2516590/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:3331439/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:3185971/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:2821540/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:2003196/69‑1 (MQ=255) aCCATCTCGCCTTTGCTAGGGTTTTCATCTTATTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:397237/69‑1 (MQ=255) ccATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:455454/68‑1 (MQ=255) ccATCTCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:3096337/68‑1 (MQ=255) cATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTg < 1:343785/69‑1 (MQ=255) cATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTg < 1:2444317/69‑1 (MQ=255) cATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTg < 1:137833/69‑1 (MQ=255) cATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTg < 1:2977454/69‑1 (MQ=255) tCGCCTTTGCTAGGGTTTTCATCTTCTTTGATCCACGCCAGCAGTTCGCCGACCGACGTGCCg < 1:60605/63‑1 (MQ=255) cTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGtt < 1:1969299/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:2473716/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:852710/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:767572/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:677633/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:1969373/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCAGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:3173322/68‑1 (MQ=255) tcttctTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCAGTGAATGGTTTCCCAGGTTTTGGTTAg < 1:650221/68‑1 (MQ=255) | TGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGACGGTTTTCATCTTCCTTTACCCACGCCAGCAGCTCGCCAACGGGCGCGCCGTGAATGGTTTCCCAGGTTTTGGTCAG > minE/2117710‑2117831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |