| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,122,104 | C→A | 28.5% | intergenic (+33/+81) | yraP → / ← yraQ | hypothetical protein/predicted permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,122,104 | 0 | C | A | 28.5% | 132.3 / 48.6 | 91 | intergenic (+33/+81) | yraP/yraQ | hypothetical protein/predicted permease |
| Reads supporting (aligned to +/- strand): ref base C (40/25); new base A (9/17); total (49/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
CGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAT > minE/2122039‑2122164 | cGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg > 1:968474/1‑69 (MQ=255)cGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg > 1:2382637/1‑69 (MQ=255)cGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg > 1:2449500/1‑69 (MQ=255)cGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg > 1:1630521/1‑69 (MQ=255)cGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg > 1:142835/1‑69 (MQ=255) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:725578/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:1910455/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2670112/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2618662/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:750544/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2850775/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:829008/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:3047725/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:365835/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:507930/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2441750/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:1526564/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2393759/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:825095/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:2298668/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:593634/69‑1 (MQ=38) ggTAACTACGGCGTTTACGTTTATTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCt < 1:61284/69‑1 (MQ=38) gCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:1631147/69‑1 (MQ=255) gCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:2892143/69‑1 (MQ=255) gCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:672239/69‑1 (MQ=255) gCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:1898642/69‑1 (MQ=255) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2485855/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2840175/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2807964/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2811926/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2816663/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:2823748/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:523639/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:1503075/1‑69 (MQ=21) tacgtttaTTAAATAGCAG‑CCCTTGTAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAc > 1:1424804/1‑69 (MQ=21) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:2741980/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:2737845/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:855760/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:2386239/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:1068275/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:1083570/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:1371224/68‑1 (MQ=255) tAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:1601892/68‑1 (MQ=255) gTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 1:251922/56‑1 (MQ=255) tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCg > 1:21561/1‑68 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCTTAGGTCAGATAAGGCGttt > 1:1590723/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:35188/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:170833/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2878169/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1659332/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2905808/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:23644/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2973060/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2976917/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:3106398/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:3285370/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:3350021/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2907771/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:674770/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1250255/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:773875/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:837740/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:946746/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1030179/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1755711/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2287663/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2124976/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2099067/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2796905/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2767876/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1907027/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1940030/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:1753468/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGttt > 1:2668276/1‑69 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGtt > 1:1157982/1‑68 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGtt > 1:250291/1‑68 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc > 1:390504/1‑68 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc > 1:125058/1‑68 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc > 1:2533517/1‑68 (MQ=255) tGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc > 1:1890447/1‑68 (MQ=255) gCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:1780945/69‑1 (MQ=255) gCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:1531513/69‑1 (MQ=255) gCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:1261367/69‑1 (MQ=255) gCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:2434041/69‑1 (MQ=255) gCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:2749247/69‑1 (MQ=255) cGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:2052635/68‑1 (MQ=255) aCGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt > 1:2041814/1‑69 (MQ=255) aCGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt > 1:1251851/1‑69 (MQ=255) aCGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt > 1:291073/1‑69 (MQ=255) aCGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt > 1:247162/1‑69 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:2348367/35‑1 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:1781399/35‑1 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:1099307/35‑1 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:3300417/35‑1 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:1565443/35‑1 (MQ=255) gCTTGCCGCGTCTTATCAGGCCTACAACGATACaa < 1:3352640/35‑1 (MQ=255) | CGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAT > minE/2122039‑2122164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |